T. Rabilloud, S. Kieffer, V. Procaccio, M. Louwagie, P. L. Courchesne et al., Two-dimensional electrophoresis of human placental mitochondria and protein identification by mass spectrometry: Toward a human mitochondrial proteome, Electrophoresis, vol.30, issue.6, pp.1006-1014, 1998.
DOI : 10.1002/elps.1150190616

A. W. Bell, M. A. Ward, W. P. Blackstock, H. N. Freeman, J. S. Choudhary et al., Proteomics Characterization of Abundant Golgi Membrane Proteins, Journal of Biological Chemistry, vol.276, issue.7, pp.5152-5165, 2001.
DOI : 10.1074/jbc.M006143200

Y. Liu, M. P. Patricelli, and B. F. Cravatt, Activity-based protein profiling: The serine hydrolases, Proceedings of the National Academy of Sciences, vol.19, issue.11, pp.14694-14699, 1999.
DOI : 10.1002/elps.1150191103

C. C. Wu, M. J. Maccoss, K. E. Howell, and J. R. Yates, A method for the comprehensive proteomic analysis of membrane proteins, Nature Biotechnology, vol.21, issue.5, pp.532-538, 2003.
DOI : 10.1038/nbt819

H. Watarai, A. Hinohara, J. Nagafune, T. Nakayama, M. Taniguchi et al., Plasma membrane-focused proteomics: Dramatic changes in surface expression during the maturation of human dendritic cells, PROTEOMICS, vol.188, issue.15, pp.4001-4011, 2005.
DOI : 10.1002/pmic.200401258

A. Dupont, C. Tokarski, O. Dekeyzer, A. L. Guihot, P. Amouyel et al., Two-dimensional maps and databases of the human macrophage proteome and secretome, PROTEOMICS, vol.4, issue.6, pp.1761-1778, 2004.
DOI : 10.1002/pmic.200300691

M. Lafon-cazal, O. Adjali, N. Galeotti, J. Poncet, P. Jouin et al., Proteomic Analysis of Astrocytic Secretion in the Mouse: COMPARISON WITH THE CEREBROSPINAL FLUID PROTEOME, Journal of Biological Chemistry, vol.278, issue.27, pp.24438-24448, 2003.
DOI : 10.1074/jbc.M211980200

J. Ziegler, T. Vogt, O. Miersch, and D. Strack, Concentration of Dilute Protein Solutions Prior to Sodium Dodecyl Sulfate???Polyacrylamide Gel Electrophoresis, Analytical Biochemistry, vol.250, issue.2, pp.257-260, 1997.
DOI : 10.1006/abio.1997.2248

D. M. Sauve, D. T. Ho, and M. Roberge, Concentration of Dilute Protein for Gel Electrophoresis, Analytical Biochemistry, vol.226, issue.2, pp.382-383, 1995.
DOI : 10.1006/abio.1995.1242

A. Bensadoun and D. Weinstein, Assay of proteins in the presence of interfering materials, Analytical Biochemistry, vol.70, issue.1, pp.241-250, 1976.
DOI : 10.1016/S0003-2697(76)80064-4

T. Rabilloud, C. Adessi, A. Giraudel, and J. Lunardi, Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients, Electrophoresis, vol.246, issue.3-4, pp.307-316, 1994.
DOI : 10.1002/elps.1150180303
URL : https://hal.archives-ouvertes.fr/hal-00118181

M. Chevallet, H. Diemer, S. Luche, A. Van-dorsselaer, T. Rabilloud et al., Improved mass spectrometry compatibility is afforded by ammoniacal silver staining, PROTEOMICS, vol.72, issue.8, pp.2350-2354, 2006.
DOI : 10.1002/pmic.200500567
URL : https://hal.archives-ouvertes.fr/hal-00115854

D. N. Perkins, D. J. Pappin, D. M. Creasy, and J. S. Cottrell, Probability-based protein identification by searching sequence databases using mass spectrometry data, Electrophoresis, vol.447, issue.18, pp.3551-3567, 1999.
DOI : 10.1002/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2

E. Thouvenot, M. Lafon-cazal, E. Demettre, P. Jouin, J. Bockaert et al., The proteomic analysis of mouse choroid plexus secretome reveals a high protein secretion capacity of choroidal epithelial cells, PROTEOMICS, vol.171, issue.22, pp.5941-5952, 2006.
DOI : 10.1002/pmic.200600096

R. M. Aguilar, J. J. Bustamante, P. G. Hernandez, A. O. Martinez, and L. S. Haro, Precipitation of Dilute Chromatographic Samples (ng/ml) Containing Interfering Substances for SDS???PAGE, Analytical Biochemistry, vol.267, issue.2, pp.344-350, 1999.
DOI : 10.1006/abio.1998.3018

T. Marshall and K. M. Williams, Recovery of protein by Coomassie Brilliant Blue precipitation prior to electrophoresis, Electrophoresis, vol.136, issue.1, pp.887-888, 1992.
DOI : 10.1002/elps.11501301195

W. M. Lestourgeon and A. L. Beyer, Chapter 26 The Rapid Isolation, High-Resolution Electrophoretic Characterzation, and Purification of Nuclear Proteins, Methods Cell Biol, vol.16, pp.387-406, 1977.
DOI : 10.1016/S0091-679X(08)60115-6

U. Arnold and R. Ulbrich-hofmann, Quantitative Protein Precipitation from Guanidine Hydrochloride-Containing Solutions by Sodium Deoxycholate/Trichloroacetic Acid, Analytical Biochemistry, vol.271, issue.2, pp.197-199, 1999.
DOI : 10.1006/abio.1999.4149

M. P. Nandakumar, J. Shen, B. Raman, and M. R. Marten, Solubilization of Trichloroacetic Acid (TCA) Precipitated Microbial Proteins via NaOH for Two-Dimensional Electrophoresis, Journal of Proteome Research, vol.2, issue.1, pp.89-93, 2003.
DOI : 10.1021/pr025541x

S. Zhou, M. J. Bailey, M. J. Dunn, V. R. Preedy, and P. W. Emery, A quantitative investigation into the losses of proteins at different stages of a two-dimensional gel electrophoresis procedure, PROTEOMICS, vol.2, issue.11, pp.2739-2747, 2005.
DOI : 10.1002/pmic.200401178

. H. Zwickl, E. Traxler, S. Staettner, W. Parzefall, B. Grasl-kraupp et al., A novel technique to specifically analyze the secretome of cells and tissues, ELECTROPHORESIS, vol.325, issue.14, pp.2779-2785, 2005.
DOI : 10.1002/elps.200410387

F. Mbeunkui, O. Fodstad, and L. K. Pannell, Secretory Protein Enrichment and Analysis:?? An Optimized Approach Applied on Cancer Cell Lines Using 2D LC???MS/MS, Journal of Proteome Research, vol.5, issue.4, pp.899-906, 2006.
DOI : 10.1021/pr050375p

M. Maldi, Q. Qtof, and M. Ms, Name: name indicated in Swiss-Prot. Acc: SwissProt accession number. Mw/pI: theoretical Mw and pI calculated from the sequence %C: coverage in % of the sequence Nb pep: number of peptides matching with the predicted protein. Ident: identification method Score: Mascot score for the proposed identification Table 2: Proteins identified in the medium conditioned by dendritic cells Short name: name indicated on the figure 3. Name: name indicated in Swiss-Prot. Acc: SwissProt accession number. Mw/pI: theoretical Mw and pI calculated from the sequence. %C: coverage in % of the sequence. Nb pep: number of peptides matching with the predicted protein, Table1: Proteins identified in the medium conditioned by J774 cells Short name: name indicated on the figure 3 Ident: identification method: M= Maldi MS, Q= Qtof MS/MS. Score: Mascot score for the proposed identification