Skip to Main content Skip to Navigation
Journal articles

Role of the kinetochore/cell cycle checkpoint protein ZW10 in interphase cytoplasmic dynein function

Abstract : Zeste white 10 (ZW10) is a mitotic checkpoint protein and the anchor for cytoplasmic dynein at mitotic kinetochores, though it is expressed throughout the cell cycle. We find that ZW10 localizes to pericentriolar membranous structures during interphase and cosediments with Golgi membranes. Dominant-negative ZW10, anti-ZW10 antibody, and ZW10 RNA interference (RNAi) caused Golgi dispersal. ZW10 RNAi also dispersed endosomes and lysosomes. Live imaging of Golgi, endosomal, and lysosomal markers after reduced ZW10 expression showed a specific decrease in the frequency of minus end-directed movements. Golgi membrane-associated dynein was markedly decreased, suggesting a role for ZW10 in dynein cargo binding during interphase. We also find ZW10 enriched at the leading edge of migrating fibroblasts, suggesting that ZW10 serves as a general regulator of dynein function throughout the cell cycle.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-00130005
Contributor : Evelyne Klotz Connect in order to contact the contributor
Submitted on : Thursday, February 8, 2007 - 5:31:30 PM
Last modification on : Friday, December 18, 2020 - 6:46:04 PM

Identifiers

  • HAL Id : hal-00130005, version 1

Collections

Citation

D. Varma, D. L. Dujardin, S. A. Stehman, R. B. Vallee. Role of the kinetochore/cell cycle checkpoint protein ZW10 in interphase cytoplasmic dynein function. Journal of Cell Biology, Rockefeller University Press, 2006, 172, pp.655-62. ⟨hal-00130005⟩

Share

Metrics

Record views

243