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DNA helicase activity is associated with the replication initiator protein rep of tomato yellow leaf curl geminivirus.

Abstract : The Rep protein of tomato yellow leaf curl Sardinia virus (TYLCSV), a single-stranded DNA virus of plants, is the replication initiator essential for virus replication. TYLCSV Rep has been classified among ATPases associated with various cellular activities (AAA+ ATPases), in superfamily 3 of small DNA and RNA virus replication initiators whose paradigmatic member is simian virus 40 large T antigen. Members of this family are DNA- or RNA-dependent ATPases with helicase activity necessary for viral replication. Another distinctive feature of AAA+ ATPases is their quaternary structure, often composed of hexameric rings. TYLCSV Rep has ATPase activity, but the helicase activity, which is instrumental in further characterization of the mechanism of rolling-circle replication used by geminiviruses, has been a longstanding question. We present results showing that TYLCSV Rep lacking the 121 N-terminal amino acids has helicase activity comparable to that of the other helicases: requirements for a 3' overhang and 3'-to-5' polarity of unwinding, with some distinct features and with a minimal AAA+ ATPase domain. We also show that the helicase activity is dependent on the oligomeric state of the protein.
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https://hal.archives-ouvertes.fr/hal-00121908
Contributor : Pascale Le Roux <>
Submitted on : Friday, December 22, 2006 - 2:23:43 PM
Last modification on : Thursday, June 25, 2020 - 1:54:09 PM

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Danielle Clérot, Françoise Bernardi. DNA helicase activity is associated with the replication initiator protein rep of tomato yellow leaf curl geminivirus.. Journal of Virology, American Society for Microbiology, 2006, 80 (22), pp.11322-30. ⟨10.1128/JVI.00924-06⟩. ⟨hal-00121908⟩

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