GABA controls the level of quorum-sensing signal in Agrobacterium tumefaciens.

Abstract : The concentration of GABA increases rapidly in wounded plant tissues, but the implication of this GABA pulse for plant-bacteria interactions is not known. Here we reveal that GABA stimulated the inactivation of the N-(3-oxooctanoyl)homoserine lactone (OC8-HSL) quorum-sensing signal (or "quormone") by the Agrobacterium lactonase AttM. GABA induced the expression of the attKLM operon, which was correlated to a decrease in OC8-HSL concentration in Agrobacterium tumefaciens cultures. The Agrobacterium GABA transporter Bra was required for this GABA-signaling pathway. Furthermore, transgenic tobacco plants with elevated GABA levels were less sensitive to A. tumefaciens C58 infection than were wild-type plants. These findings indicate that plant GABA may modulate quorum sensing in A. tumefaciens, thereby affecting its virulence on plants. Whereas GABA is an essential cell-to-cell signal in eukaryotes, here we provide evidence of GABA acting as a signal between eukaryotes and pathogenic bacteria. The GABA signal represents a potential target for the development of a strategy to control the virulence of bacterial pathogens.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-00119068
Contributor : Pascale Le Roux <>
Submitted on : Thursday, December 7, 2006 - 3:40:15 PM
Last modification on : Tuesday, October 29, 2019 - 8:36:09 PM

Links full text

Identifiers

Collections

Citation

Romain Chevrot, Ran Rosen, Elise Haudecoeur, Amélie Cirou, Barry J Shelp, et al.. GABA controls the level of quorum-sensing signal in Agrobacterium tumefaciens.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2006, 103 (19), pp.7460-4. ⟨10.1073/pnas.0600313103⟩. ⟨hal-00119068⟩

Share

Metrics

Record views

100