Precraniate origin of cranial motoneurons.

Héloïse D Dufour (1) , Zoubida Chettouh (1) , Carole Deyts (2, 3, 4, 5) , Renaud de Rosa (1) , Christo Goridis (1) , Jean-Stéphane Joly (2, 3, 4) , Jean-François Brunet (1)

1 DESN - Développement et évolution du système nerveux
2 DEPSN - Développement, évolution et plasticité du système nerveux
3 INAF - Institut de Neurobiologie Alfred Fessard
4 INRA MSNC group Institut Fessard, CNRS
5 NPA - Neurobiologie des processus adaptatifs

The craniate head is innervated by cranial sensory and motor neurons. Cranial sensory neurons stem from the neurogenic placodes and neural crest and are seen as evolutionary innovations crucial in fulfilling the feeding and respiratory needs of the craniate "new head." In contrast, cranial motoneurons that are located in the hindbrain and motorize the head have an unclear phylogenetic status. Here we show that these motoneurons are in fact homologous to the motoneurons of the sessile postmetamorphic form of ascidians. The motoneurons of adult Ciona intestinalis, located in the cerebral ganglion and innervating muscles associated with the huge "branchial basket," express the transcription factors CiPhox2 and CiTbx20, whose vertebrate orthologues collectively define cranial motoneurons of the branchiovisceral class. Moreover, Ciona's postmetamorphic motoneurons arise from a hindbrain set aside during larval life and defined as such by its position (caudal to the prosensephalic sensory vesicle) and coexpression of CiPhox2 and CiHox1, whose orthologues collectively mark the vertebrate hindbrain. These data unveil that the postmetamorphic ascidian brain, assumed to be a derived feature, in fact corresponds to the vertebrate hindbrain and push back the evolutionary origin of cranial nerves to before the origin of craniates.

Mots clés: Animals; Embryo; Ganglion Cysts; Head; Homeodomain Proteins; Larva; Metamorphosis; Mice; Molecular Sequence Data

Mots clés: Ciona intestinalis; Nonmammalian; Biological; Motor Neurons; T-Box Domain Proteins

Domaines: Neurosciences [q-bio.NC]

Héloïse D Dufour, Zoubida Chettouh, Carole Deyts, Renaud de Rosa, Christo Goridis, et al.. Precraniate origin of cranial motoneurons.. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103 (23), pp.8727-32. ⟨10.1073/pnas.0600805103⟩. ⟨hal-00113783⟩