Skip to Main content Skip to Navigation
Journal articles

Conserved and specific functions of axoneme components in trypanosome motility.

Abstract : The Trypanosoma brucei flagellum is unusual as it is attached along the cell body and contains, in addition to an apparently conventional axoneme, a structure called the paraflagellar rod, which is essential for cell motility. Here, we investigated flagellum behaviour in normal and mutant trypanosome cell lines where expression of genes encoding various axoneme proteins (PF16, PF20, DNAI1, LC2) had been silenced by RNAi. First, we show that the propulsive wave (normally used for forward motility) is abolished in the absence of outer dynein arms, whereas the reverse wave (normally used for changing direction) still occurs. Second, in contrast to Chlamydomonas--but like metazoa, the central pair adopts a fixed orientation during flagellum beating. This orientation becomes highly variable in central-pair- and outer-dynein-arm-mutants. Third, the paraflagellar rod contributes to motility by facilitating three-dimensional wave propagation and controlling cell shape. Fourth, motility is required to complete the last stage of cell division in both insect and bloodstream stages of the parasite. Finally, our study also reveals the conservation of molecular components of the trypanosome flagellum. Coupled to the ease of reverse genetics, it raises the interest of trypanosomes as model organisms to study cilia and flagella.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-00108209
Contributor : Philippe Bastin <>
Submitted on : Thursday, October 19, 2006 - 10:52:04 PM
Last modification on : Friday, January 15, 2021 - 11:30:03 AM
Long-term archiving on: : Tuesday, April 6, 2010 - 8:15:44 PM

Identifiers

Citation

Carole Branche, Linda Kohl, Géraldine Toutirais, Johanna Buisson, Jacky Cosson, et al.. Conserved and specific functions of axoneme components in trypanosome motility.. Journal of Cell Science, Company of Biologists, 2006, 119 (Pt 16), pp.3443-55. ⟨10.1242/jcs.03078⟩. ⟨hal-00108209⟩

Share

Metrics

Record views

567

Files downloads

713