Strain typing with ISLpl1 in lactobacilli.

Tanja Petrovic (1) , Miomir Niksic (1) , Françoise Bringel (2)

1 IFTB - institute of Food Technology
2 GMGM - Génétique moléculaire, génomique, microbiologie

Twenty-seven Lactobacillus plantarum ssp. plantarum, 11 Lactobacillus paraplantarum and five Lactobacillus casei-related strains, isolated from various autochthonous Serbian and Montenegro-fermented foods, were identified using phenotypical characterization and current PCR methods based on PCR of the recA gene or the 23S-5S rRNA gene intragenic spacer (IS) region. The strains were genotypically characterized by a new method based on the insertion sequence element ISLpl11 that grouped these lactobacilli into 10 IS-fingerprinting groups. Between six and 23 copies of the ISLpl1 were found in each strain and the ISLpl1-fingerprint groups correlated well with the origin of the strains. The method proved suitable for strain typing of lactic acid bacteria at the infraspecies level.

Domaines: Biochimie, Biologie Moléculaire

Tanja Petrovic, Miomir Niksic, Françoise Bringel. Strain typing with ISLpl1 in lactobacilli.. Microbiology Letters, 2006, 255 (1), pp.1-10. ⟨10.1111/j.1574-6968.2005.00042.x⟩. ⟨hal-00107341⟩