Chemiluminescence of enterococci isolates from freshwater.

Philippe Andre , Catherine Metzger , Sophie Petey , Dominique J-M Vidon , Daniel Muller (1)

1 GMGM - Génétique moléculaire, génomique, microbiologie

All Enterococcus spp., isolated from environmental water samples (n=81), emitted a high chemiluminescence signal in the presence of luminol (10(-2) M). Kinetic studies of chemiluminescence show a close correlation between chemiluminescence and growth curves during the exponential phase, with a maximum chemiluminescence reached just before bacterial growth entered in the stationary phase. On the other hand, genera closely related to Enterococcus such as Streptococcus or Lactococcus produced a very weak chemiluminescent signal. Chemiluminescence of enterococci could therefore offer a rapid test, in aiding the identification of the genus Enterococcus and in the survey of the microbiological quality of water supplies.

Mots clés: chemiluminescence

Domaines: Bactériologie

Philippe Andre, Catherine Metzger, Sophie Petey, Dominique J-M Vidon, Daniel Muller. Chemiluminescence of enterococci isolates from freshwater.. Microbiology Letters, 2005, 245 (1), pp.123-9. ⟨10.1016/j.femsle.2005.02.036⟩. ⟨hal-00106769⟩