Crystallization and preliminary X-ray diffraction data of an archaeal asparagine synthetase related to asparaginyl-tRNA synthetase. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Acta Crystallogr D Biol Crystallogr Année : 2004

Crystallization and preliminary X-ray diffraction data of an archaeal asparagine synthetase related to asparaginyl-tRNA synthetase.

Résumé

The archaebacterial-type asparagine synthetase A from Pyrococcus abyssi (AS-AR), which is related to asparaginyl-tRNA synthetase, was crystallized in two different conditions using the hanging-drop vapour-diffusion method. Crystals belonging to space group C2 with unit-cell parameters a = 103.6, b = 43.3, c = 121.5 A, beta = 112.6 degrees and one dimer per asymmetric unit were obtained in the presence of 2-propanol and PEG 4000 at pH 5.6. Another crystal form was obtained in the presence of dioxan and belongs to the monoclinic space group P2(1), with unit-cell parameters a = 96.8, b = 103.9, c = 98.4 A, beta = 107.5 degrees and two dimers per asymmetric unit. Two different native diffraction data sets were collected to 2.3 and 3.0 A resolution using synchrotron radiation and cryocooling for crystals belonging to space groups C2 and P2(1), respectively.

Dates et versions

hal-00105494 , version 1 (11-10-2006)

Identifiants

Citer

Christophe Charron, Hervé Roy, Mickaël Blaise, Richard Giegé, Daniel Kern. Crystallization and preliminary X-ray diffraction data of an archaeal asparagine synthetase related to asparaginyl-tRNA synthetase.. Acta Crystallogr D Biol Crystallogr, 2004, 60 (Pt 4), pp.767-9. ⟨10.1107/S0907444904003117⟩. ⟨hal-00105494⟩

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