Speciation of non-covalent nickel species in plant tissue extracts by electrospray Q-TOFMS/MS after their isolation by 2D size exclusion-hydrophilic interaction LC (SEC-HILIC) monitored by ICP-MS

Laurent Ouerdane (1) , S. Mari (2) , P. Czernic (2) , M. Lebrun (2) , Ryszard Lobinski (1, 3)

1 LCABIE - Laboratoire de Chimie Analytique Bio-Inorganique et Environnement
2 BPMP - Biochimie et Physiologie Moléculaire des Plantes
3 Faculty of Chemistry

An original approach based on successive size-exclusion and hydrophilic interaction HPLC (HILIC) was developed to purify traces of Ni species from a plant aqueous extract. The degree of purity achieved was for the first time sufficient for the identification, in a natural sample, of a number of non-covalent metal complexes by electrospray Q-TOFMS/MS. Nickel complexes with malate, citrate, histidine, EDTA and nicotianamine (NA) were identified in the roots, xylem, shoots and their protoplasts of a metal hyperaccumulator plant Thlaspi caerulescens. The quantitative recovery of the most stable of these complexes (with EDTA and NA) allowed their quantitative determination by SEC-ICP-MS.

Mots clés: hyperaccumulator thlaspi-caerulescens; performance liquid-chromatography; arsenic-phytochelatin complexes; mass-spectrometry; arabidopsis-halleri; polar compounds; metal hyperaccumulation; transgenic plants; zinc; phytoremediation

Domaines: Biologie végétale

Laurent Ouerdane, S. Mari, P. Czernic, M. Lebrun, Ryszard Lobinski. Speciation of non-covalent nickel species in plant tissue extracts by electrospray Q-TOFMS/MS after their isolation by 2D size exclusion-hydrophilic interaction LC (SEC-HILIC) monitored by ICP-MS. Journal of Analytical Atomic Spectrometry, 2006, 21 (7), pp.676-683. ⟨10.1039/b602689c⟩. ⟨hal-00098200⟩