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Article Dans Une Revue BBA - Biochimica et Biophysica Acta Année : 2006

Development of a low-pressure diamond anvil cell and analytical tools to monitor microbial activities in situ under controlled P and T

Résumé

We have designed a new low-pressure Diamond Anvil Cell (DAC), calibrated two novel pressure calibrants and validated the use of semi-quantitative Raman and Xray spectroscopies to monitor the fate of microbes, their metabolism or their cellular components under controlled pressures and temperatures in the 0.1-1.4 GPa and 20°-300°C P,T range. The low-pressure DAC has a 250-600 µm thick observation diamond window to allow for lower detection limits and improved microscopic imaging. This new design allows the determination of cellular growth parameters from automated image analysis, which can be correlated with the spectroscopic data obtained on metabolism, ensuring high quality data collection on microbial activity under pressure. The novel pressure sensors offer the ease of use of the well-known ruby scale, while being more sensitive and reacting to pressure variations instantaneously.

Domaines

Bactériologie
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Dates et versions

hal-00093586 , version 1 (13-09-2006)

Identifiants

Citer

Philippe M. Oger, Isabelle Daniel, Aude Picard. Development of a low-pressure diamond anvil cell and analytical tools to monitor microbial activities in situ under controlled P and T. BBA - Biochimica et Biophysica Acta, 2006, 1764, pp.434-442. ⟨10.1016/j.bbapap.2005.11.009⟩. ⟨hal-00093586⟩
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