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Article Dans Une Revue Analytical Chemistry Année : 2007

Molecular profiling of matrix coated and native tissue sections by UV and IR MALDI-O-TOF-MS.

Résumé

Phospho- and glycolipids contained in the plasma membrane of neuronal tissue were profiled by direct infrared laser desorption/ionization orthogonal time-of-flight mass spectrometry (IR-LDI-o-TOF-MS), performed on cryosected native slices generated from rat brain. About 100 different detected lipid species are putatively assigned based on their molecular weight. Spraying of potassium acetate onto the slices was found to facilitate data interpretation in positive ion mode by reducing residual sodium adduct ion intensities. Coating the slices with matrix and using an ultraviolet laser for UV-MALDI-o-TOF-MS extends the analysis to peptides and small proteins but induces analyte diffusion. Peptides and partially cleaved proteins derived from proteolytic digests were recorded after incubation of native sections with trypsin and subsequent coating of the slices with MALDI matrix
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Dates et versions

hal-00086947 , version 1 (18-08-2007)

Identifiants

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Klaus Dreisewerd, R. Lemaire, G. Pohlentz, M. Salzet, M. Wisztorski, et al.. Molecular profiling of matrix coated and native tissue sections by UV and IR MALDI-O-TOF-MS.. Analytical Chemistry, 2007, 79 (6), pp.2463 -2471. ⟨10.1021/ac061768q⟩. ⟨hal-00086947⟩

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