In silico identification and structural features of six new genes similar to MATER specifically expressed in the oocyte.

Sébastien Dadé (1) , Isabelle Callebaut (2) , Amélie Paillisson (1) , Martine Bontoux (1) , Rozenn Dalbies-Tran (1) , Philippe Monget (1)

1 PRC - Physiologie de la reproduction et des comportements [Nouzilly]
2 IMPMC - Institut de minéralogie et de physique des milieux condensés

In the present work, we have used the in silico subtraction methodology to identify six new mouse genes similar to NALP5/MATER, whose ESTs were represented almost exclusively in egg libraries. Five genes were selected for RT-PCR and/or in situ hybridization. These experiments confirmed their oocyte restricted expression. Five of these genes are localized on mouse chromosome 7, as is NALP5/MATER; among them, three are localized in a 300 kb cluster.

Mots clés: In silico; Oocyte; Mouse; Expression; MATER

Domaines: Sciences du Vivant [q-bio]; Informatique [cs]

Sébastien Dadé, Isabelle Callebaut, Amélie Paillisson, Martine Bontoux, Rozenn Dalbies-Tran, et al.. In silico identification and structural features of six new genes similar to MATER specifically expressed in the oocyte.. Biochemical and Biophysical Research Communications, 2004, 324, pp.547-553. ⟨10.1016/j.bbrc.2004.09.086⟩. ⟨hal-00086729⟩