Lipid bilayer tethered inside a nanoporous support : a solid-state NMR investigation

Olivier Wattraint (1) , Alexandre Arnold (2) , Michèle Auger (2) , Christian Bourdillon (1) , Catherine Sarazin (1)

1 GEC - Génie Enzymatique et Cellulaire
2 Centre de Recherche en Sciences et Ingénierie des Macromolécules

31P and 1H solid-state nuclear magnetic resonance (NMR) experiments have been designed with the aim of studying directly the formation of supported bilayers tethered inside nanoporous aluminum oxide supports as a model of biomimetic membranes. The static and magic angle spinning 31P NMR spectra of the supported bilayers have been compared with the experimental and simulated spectra of a simpler model with cylindrical geometry, namely a phospholipid bilayer adsorbed on an oriented polymer sheet. The broadening observed for the nanoporous model is most likely due to the presence of paramagnetic ions in the aluminum oxide. A phospholipid lateral diffusion coefficient of (2.8 ± 0.4) × 10?8 cm2/s has been measured for the tethered bilayer on a spherical support, indicating a good fluidity as compared with adsorbed membrane models.

Domaines: Chimie

Olivier Wattraint, Alexandre Arnold, Michèle Auger, Christian Bourdillon, Catherine Sarazin. Lipid bilayer tethered inside a nanoporous support : a solid-state NMR investigation. Analytical Biochemistry, 2005, 336 (2), pp.253-261. ⟨10.1016/j.ab.2004.09.041⟩. ⟨hal-00071222⟩