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An efficient procedure for purification of the obligate intracellular Wolbachia pipientis and representative amplification of its genome by multiple displacement amplification

Patrick Mavingui 1 Van Tran Van 1 Estelle Labeyrie 1 Edwige Rancès 1 Fabrice Vavre 2 Pascal Simonet 1
2 HELIX - Computer science and genomics
Inria Grenoble - Rhône-Alpes, LBBE - Laboratoire de Biométrie et Biologie Evolutive - UMR 5558
Abstract : procedure to representatively amplify Wolbachia genome by multiple displacement amplification from limited infected host tissue (0.2 g or 2 x 107 cell line). We obtained sufficient amounts (8 to 10 µg) of DNA of suitable quality for genomic studies with which we demonstrated that the amplified DNA contained all of the Wolbachia loci targeted. In addition, our data indicated that the genome of wRi strain, an obligatory endosymbiont of Drosophila simulans, shared similar overall architecture with its relative strain wMel.
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https://hal.archives-ouvertes.fr/hal-00023397
Contributor : Christine Delorme <>
Submitted on : Monday, November 13, 2006 - 4:46:53 PM
Last modification on : Wednesday, July 21, 2021 - 3:49:50 AM

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  • HAL Id : hal-00023397, version 1
  • PRODINRA : 251979

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Patrick Mavingui, Van Tran Van, Estelle Labeyrie, Edwige Rancès, Fabrice Vavre, et al.. An efficient procedure for purification of the obligate intracellular Wolbachia pipientis and representative amplification of its genome by multiple displacement amplification. Applied and Environmental Microbiology, American Society for Microbiology, 2005, 71, pp.6910-6917. ⟨hal-00023397⟩

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