Use of coherent control for selective two-photon fluorescence microscopy in live organisms.

Abstract : We demonstrate selective fluorescence We demonstrate selective fluorescence excitation of specific molecular species in live organisms by using coherent control of two-photon excitation. We have acquired quasi-simultaneous images in live fluorescently-labeled Drosophila embryos by rapid switching between appropriate pulse shapes. Linear combinations of these images demonstrate that a high degree of fluorophore selectivity is attainable through phase-shaping. Broadband phase-shaped excitation opens up new possibilities for single-laser, multiplex, in-vivo fluorescence microscopy. © 2006 Optical Society of America
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Jennifer Ogilvie, Delphine Débarre, Xavier Solinas, Jean-Louis Martin, Emmanuel Beaurepaire, et al.. Use of coherent control for selective two-photon fluorescence microscopy in live organisms.. Optics Express, Optical Society of America, 2006, 14 (2), pp.759-766. ⟨10.1364/OPEX.14.000759⟩. ⟨hal-00018766⟩

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