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Wave front engineering for microscopy of living cells.

Abstract : A new method to perform simultaneously three dimensional optical sectioning and optical manipulation is presented. The system combines a multi trap optical tweezers with a video microscope to enable axial scanning of living cells while maintaining the trapping configuration at a fixed position. This is achieved compensating the axial movement of the objective by shaping the wave front of the trapping beam with properly diffractive optical elements displayed on a computer controlled spatial light modulator. Our method has been validated in three different experimental configurations. In the first, we decouple the position of a trapping plane from the axial movements of the objective and perform optical sectioning of a circle of beads kept on a fixed plane. In a second experiment, we extend the method to living cell microscopy by showing that mechanical constraints can be applied on the dorsal surface of a cell whilst performing its fluorescence optical sectioning. In the third experiment, we trapped beads in a three dimensional geometry and perform, always through the same objective, an axial scan of the volume delimited by the beads.
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https://hal.archives-ouvertes.fr/hal-00014507
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Submitted on : Friday, November 25, 2005 - 4:34:01 PM
Last modification on : Friday, November 27, 2020 - 12:36:02 PM
Long-term archiving on: : Friday, April 2, 2010 - 11:12:30 PM

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Valentina Emiliani, Dan Cojoc, Enrico Ferrari, Valeria Garbin, Christiane Durieux, et al.. Wave front engineering for microscopy of living cells.. Optics Express, Optical Society of America - OSA Publishing, 2005, 13, pp.1395-1405. ⟨hal-00014507⟩

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