Plant Kin and Kout channels: Approaching the trait of opposite 3 rectification by analyzing more than 250 KAT1–SKOR chimeras
Résumé
Members of the Shaker-like plant K+ channel family share a common structure, but are highly diverse in their function: they behave as either hyperpolarization-activated inward-rectifying (Kin) channels, or leak-like (Kweak) channels, or depolarization-activated outward-rectifying (Kout) channels. Here we created 256 chimeras between the Kin channel KAT1 and the Kout channel SKOR. The chimeras were screened in a potassium-uptake de.cient yeast strain to identify those, which mediate potassium inward currents, i.e., which are functionally equivalent to KAT1. This strategy allowed us to identify three chimeras which di.er from KAT1 in three parts of the polypeptide: the cytosolic N-terminus, the cytosolic C-terminus, and the putative voltage-sensor S4. Additionally, mutations in the Kout channel SKOR were generated in order to localize molecular entities underlying its depolarization activation. The triple mutant SKOR-D312N-M313L-I314G, carrying amino-acid changes in the S6 segment, was identied as a channel which did not display any recti.cation in the tested voltage-range.