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Weak Effect of Membrane Diffusion on the Rate of Receptor Accumulation at Adhesive Contacts.

Abstract : To assess if membrane diffusion could affect the kinetics of receptor recruitment at adhesive contacts, we transfected neurons with GFP-tagged IgCAMs of varying length (25-180 kD), and measured the lateral mobility of single Quantum Dots bound to those receptors at the cell surface. The diffusion coefficient varied within a physiological range (0.1-0.5 µm2/s), and was inversely proportional to the size of the receptor. We then triggered adhesive contact formation by placing anti-GFP coated microspheres on growth cones using optical tweezers, and measured surface receptor recruitment around microspheres by time-lapse fluorescence imaging. The accumulation rate was rather insensitive to the type of receptor, suggesting that the long range membrane diffusion of IgCAMs is not a limiting step in the initiation of neuronal contacts.
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https://hal.archives-ouvertes.fr/hal-00009552
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Submitted on : Wednesday, October 5, 2005 - 11:22:03 AM
Last modification on : Friday, April 10, 2020 - 5:06:40 PM
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Olivier Thoumine, Edouard Saint-Michel, Caroline Dequidt, Julien Falk, Rachel Rudge, et al.. Weak Effect of Membrane Diffusion on the Rate of Receptor Accumulation at Adhesive Contacts.. Biophysical Journal, Biophysical Society, 2005, 89(5), pp.L40-42. ⟨10.1529/biophysj.105.071688⟩. ⟨hal-00009552⟩

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