Mapping candidate genes in Eucalyptus with emphasis on lignification genes

Jean-Marc Gion (1) , Philippe Rech (1) , Jacqueline Grima Pettenati (2) , Daniel Verhaegen (1) , Christophe Plomion (3)

1 Cirad-FORET - Département Forêt
2 UT3 - Université Toulouse III - Paul Sabatier
3 Laboratoire de Génétique et Amélioration des Arbres Forestiers

e used the single-strand conformation polymorphism (SSCP) technique to map eight genes on Eucalyptus uro- phylla and Eucalyptus grandis linkage maps. These included four genes involved in the common phenylpropanoid pathway (caffeic acid 3-0-methyltransferase, caffeoyl CoA 3-O-methyltransferase, 4-coumarate CoA ligase and phenylalanine ammonia-lyase), two genes involved in the 'lignin specific' pathway (cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase), and two symbiosis regulated genes (EgHypar and EgTubA1). A novel source of variation which affects the SSCP pattern, i.e. the presence or absence of electrophoresis buffer upon loading the samples into the polyacrylamide gel, was found. The placement of these genes on the Eucalyptus maps was carried out using an interspecific hybrid mapping population. This will further facilitate the identification or exclusion of 'positional' candidate genes for characterizing quantitative trait loci (QTL) for wood quality and vegetative propagation related traits

Mots clés: candidate gene; Eucalyptus; lignins; mapping; single-strand conformation polymorphism

Domaines: Biodiversité et Ecologie

Jean-Marc Gion, Philippe Rech, Jacqueline Grima Pettenati, Daniel Verhaegen, Christophe Plomion. Mapping candidate genes in Eucalyptus with emphasis on lignification genes. Molecular Breeding, 2000, 6, pp.441-449. ⟨cirad-00845442⟩