, the VENOMICS project, European project grant N° 278346 (FP7 HEALTH, 2011.
, Gif sur Yvette F-91191, France. 2 Architecture et Fonction des Macromolécules Biologiques (A.F.M.B.), UMR7257 CNRS, vol.932
,
Venoms as a platform for human drugs: translating toxins into therapeutics, Expert Opin Biol Ther, vol.11, pp.1469-1484, 2011. ,
Ziconotide: Neuronal calcium channel blocker for treating severe chronic pain, Curr Med Chem, vol.11, pp.3029-3040, 2004. ,
Rational design of a CD4 mimic that inhibits HIV-1 entry and exposes cryptic neutralization epitopes, Nat Biotechnol, vol.21, pp.71-76, 2003. ,
Small binding proteins selected from a combinatorial repertoire of knottins displayed on phage, J Mol Biol, vol.277, pp.317-332, 1998. ,
Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies, Nat Biotechnol, vol.24, pp.1551-1557, 2006. ,
Engineered knottin peptides: a New class of agents for imaging integrin expression in living subjects, Cancer Res, vol.69, pp.2435-2442, 2009. ,
Production of recombinant proteins by microbes and higher organisms, Biotechnol Adv, vol.27, pp.297-306, 2009. ,
Dual expression system suitable for high-throughput fluorescence-based screening and production of soluble proteins, J Proteome Res, vol.4, pp.2137-2147, 2005. ,
Highthroughput protein expression screening and purification in Escherichia coli, Methods, vol.55, pp.65-72, 2011. ,
MARINE-EXPRESS: taking advantage of high throughput cloning and expression strategies for the post-genomic analysis of marine organisms, Microb Cell Fact, vol.9, p.45, 2010. ,
URL : https://hal.archives-ouvertes.fr/hal-00609166
The high-throughput protein sample production platform of the Northeast Structural Genomics Consortium, J Struct Biol, vol.172, pp.21-33, 2010. ,
A new system for expressing recombinant animal toxins in E. coli, Advances and new technologies in toxinology. France: Collection Rencontres en Toxinologie, Publications de la SFET, vol.2010, pp.149-152 ,
Screening of fusion partners for high yield expression and purification of bioactive viscotoxins, Protein Expr Purif, vol.64, pp.16-23, 2009. ,
Expression of a cytotoxic cationic antibacterial peptide in Escherichia coli using two fusion partners, Protein Expr Purif, vol.57, pp.303-311, 2008. ,
Functional expression of a recombinant toxin -rPnTx2-6 -active in erectile function in rat, Toxicon, vol.56, pp.1172-1180, 2010. ,
In vitro production of three-finger neurotoxins from snake venoms, a disulfide rich proteins. Problems and their solutions (Review), Russ J Bioorg Chem, vol.36, pp.137-145, 2010. ,
Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli, Microb Cell Fact, vol.8, p.26, 2009. ,
Reexamination of the folding of BPTI: predominance of native intermediates, Science, vol.253, pp.1386-1393, 1991. ,
The disulfide folding pathway of BPTI, Science, vol.256, pp.111-114, 1992. ,
The disulfide folding pathway of tick anticoagulant peptide (TAP), a Kunitz-type inhibitor structurally homologous to BPTI, Biochemistry, vol.35, pp.11702-11709, 1996. ,
Oxidative folding and structural analyses of a kunitz-related inhibitor and its disulfide intermediates: functional implications, J Mol Biol, vol.414, pp.427-441, 2011. ,
Native folding of aggregation-prone recombinant proteins in Escherichia coli by osmolytes, plasmid-or benzyl alcohol-overexpressed molecular chaperones, Cell Stress Chaperones, vol.10, pp.329-339, 2005. ,
Recombinant protein folding and misfolding in Escherichia coli, Nat Biotechnol, vol.22, pp.1399-1408, 2004. ,
A dynamic pharmacophore drives the interaction between Psalmotoxin-1 and the putative drug target acidsensing ion channel 1a, Mol Pharmacol, vol.80, pp.796-808, 2011. ,
Functional expression in escherichia coli of the disulfide-rich sea anemone peptide APETx2, a potent blocker of acid-sensing Ion channel 3, vol.10, pp.1605-1618, 2012. ,
The name's bond......disulfide bond, Curr Opin Struct Biol, vol.17, pp.691-698, 2007. ,
The disulfide bond formation (Dsb) system, Curr Opin Struct Biol, vol.18, pp.450-458, 2008. ,
Tuned Escherichia coli as a host for the expression of disulfide-rich proteins, Biotechnol J, vol.6, pp.686-699, 2011. ,
Secretory production of recombinant proteins in escherichia coli, Recent Pat Biotechnol, vol.4, pp.23-29, 2010. ,
Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm, Proc Natl Acad Sci, vol.96, pp.13703-13708, 1999. ,
Mutations that allow disulfide bond formation in the cytoplasm of Escherichia-coli, Science, vol.262, pp.1744-1747, 1993. ,
Heterologous expression of lipase in Escherichia coli is limited by folding and disulfide bond formation, Appl Microbiol Biotechnol, vol.81, pp.79-87, 2008. ,
Enhancement of recombinant soluble dengue virus 2 envelope domain III protein production in Escherichia coli trxB and gor double mutant, J Biosci Bioeng, vol.102, pp.333-339, 2006. ,
Functional plasticity of a peroxidase allows evolution of diverse disulfide-reducing pathways, Proc Natl Acad Sci, vol.105, pp.6735-6740, 2008. ,
Expression of active human sialyltransferase ST6GalNAcI in Escherichia coli, Microb Cell Fact, vol.8, p.50, 2009. ,
SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm, Microb Cell Fact, vol.11, p.56, 2012. ,
Board of Regents: Compositions and methods for production of disulfide bond containing proteins in host cells, 2008. ,
Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli, Microb Cell Fact, vol.4, p.1, 2005. ,
Enhancement of soluble protein expression through the use of fusion tags, Curr Opin Biotechnol, vol.17, pp.353-358, 2006. ,
Tagging recombinant proteins to enhance solubility and aid purification, Methods Mol Biol, vol.681, pp.151-175, 2011. ,
Single-step purification of polypeptides expressed in escherichia-coli as fusions with glutathione S-transferase, Gene, vol.67, pp.31-40, 1988. ,
An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein, Gene, vol.74, pp.365-373, 1988. ,
High-level production and isotope labeling of snake neurotoxins, disulfide-rich proteins, Protein Expr Purif, vol.10, pp.293-300, 1997. ,
Design of an expression system for detecting folded protein domains and mapping macromolecular interactions by NMR, Protein Sci, vol.6, pp.2359-2364, 1997. ,
Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression, BMC Biotechnol, vol.4, p.32, 2004. ,
Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli, J Mol Biol, vol.320, pp.1-10, 2002. ,
Disulfide bond isomerization in prokaryotes, Biochim Biophys Acta, vol.1783, pp.530-534, 2008. ,
Many paths to many clones: a comparative look at high-throughput cloning methods, Genome Res, vol.14, pp.2020-2028, 2004. ,
High-throughput expression screening and purification of recombinant proteins in E. coli, Methods Mol Biol, 2013. ,
Protein production by auto-induction in high density shaking cultures, Protein Expr Purif, vol.41, pp.207-234, 2005. ,
Identification of various allosteric interaction sites on M1 muscarinic receptor using 125I-Met35-oxidized muscarinic toxin 7, Mol Pharmacol, vol.69, pp.1641-1651, 2006. ,
Infestin, a thrombin inhibitor presents in Triatoma infestans midgut, a Chagas' disease vector: gene cloning, expression and characterization of the inhibitor, Insect Biochem Mol Biol, vol.32, pp.991-997, 2002. ,
Knottin cyclization: impact on structure and dynamics, BMC Struct Biol, vol.8, p.54, 2008. ,
URL : https://hal.archives-ouvertes.fr/hal-00389417
The three-dimensional structure of recombinant leech-derived tryptase inhibitor in complex with trypsin -Implications for the structure of human mast cell tryptase and its inhibition, J Biol Chem, vol.272, pp.19931-19937, 1997. ,
Substitutions at the P-1 ' position in BPTI strongly affect the association energy with serine proteinases, J Mol Biol, vol.301, pp.205-217, 2000. ,
Production of disulfide-bonded proteins in Escherichia coli, Protein Expr Purif, vol.82, pp.240-251, 2012. ,
Recombinant expression and affinity purification of a novel epididymal human sperm-binding protein, BSPH1, Mol Hum Reprod, vol.15, pp.105-114, 2009. ,
Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconventional use of oxidizing environments with a maltose binding protein-hexahistine dual tag to enhance membrane protein yield, Microb Cell Fact, vol.10, p.51, 2011. ,
Disulfide bond formation in the cytoplasm, Antioxid Redox Signal, 2012. ,
Solubility of disulfide-bonded proteins in the cytoplasm of Escherichia coli and its "oxidizing" mutant, World J Gastroenterol, vol.11, pp.1077-1082, 2005. ,
Disruption of reducing pathways is not essential for efficient disulfide bond formation in the cytoplasm of, E. coli. Microb Cell Fact, vol.9, p.67, 2010. ,
Preexpression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of, E.coli. Microb Cell Fact, vol.10, p.1, 2011. ,
Functional significance of the betahairpin in the insecticidal neurotoxin omega-atracotoxin-Hv1a, J Biol Chem, vol.276, pp.26568-26576, 2001. ,
High yield expression and purification of isotopically labelled human endothelin-1 for use in NMR studies, Protein Expr Purif, vol.48, pp.253-260, 2006. ,
A novel expression system for production of soluble prion proteins in, E. coli. Microb Cell Fact, vol.11, p.6, 2012. ,
Small-molecule diselenides catalyze oxidative protein folding in vivo, ACS Chem Biol, vol.5, pp.177-182, 2010. ,
Ultra-high performance liquid chromatography-mass spectrometry for the fast profiling of histone post-translational modifications, J Proteome Res, vol.9, pp.5501-5509, 2010. ,
An accurate method for determination of receptorligand and enzyme-inhibitor dissociation constants from displacement curves, Proc Natl Acad Sci, vol.84, pp.6654-6658, 1987. ,
High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in, E. coli. Microbial Cell Factories, vol.12, p.37, 2013. ,
URL : https://hal.archives-ouvertes.fr/cea-01989636