Validation approach for a fast and simple targeted screening method for 75 antibiotics in meat and aquaculture products using LC-MS/MS - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment Année : 2016

Validation approach for a fast and simple targeted screening method for 75 antibiotics in meat and aquaculture products using LC-MS/MS

Résumé

An approach is described to validate a fast and simple targeted screening method for antibiotic analysis in meat and aquaculture products by LC-MS/MS. The strategy of validation was applied for a panel of 75 antibiotics belonging to different families, i.e., penicillins, cephalosporins, sulfonamides, macrolides, quinolones and phenicols. The samples were extracted once with acetonitrile, concentrated by evaporation and injected into the LC-MS/MS system. The approach chosen for the validation was based on the Community Reference Laboratory (CRL) guidelines for the validation of screening qualitative methods. The aim of the validation was to prove sufficient sensitivity of the method to detect all the targeted antibiotics at the level of interest, generally the maximum residue limit (MRL). A robustness study was also performed to test the influence of different factors. The validation showed that the method is valid to detect and identify 73 antibiotics of the 75 antibiotics studied in meat and aquaculture products at the validation levels.
Fichier non déposé

Dates et versions

anses-01419484 , version 1 (19-12-2016)

Identifiants

Citer

Estelle Dubreil-Cheneau, Sophie Gautier, Marie-Pierre Fourmond, Mélaine Bessiral, Murielle Gaugain-Juhel, et al.. Validation approach for a fast and simple targeted screening method for 75 antibiotics in meat and aquaculture products using LC-MS/MS. Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment, 2016, pp.1-16. ⟨10.1080/19440049.2016.1230278⟩. ⟨anses-01419484⟩

Collections

ANSES
79 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More