Skip to Main content Skip to Navigation
Journal articles

A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation

Abstract : Abstract The development of livers-on-a-chip aims to provide pharmaceutical companies with reliable systems to perform drug screening and toxicological studies. To that end, microfluidic systems are engineered to mimic the functions and architecture of this organ. In this context we have designed a device that reproduces series of liver microarchitectures, each permitting the 3D culture of hepatocytes by confining them to a chamber that is separated from the medium conveying channel by very thin slits. We modified the structure to ensure its compatibility with the culture of hepatocytes from different sources. Our device was adapted to the migratory and adhesion properties of the human HepaRG cell line at various stages of differentiation. Using this device, it was possible to keep the cells alive for more than 14 days, during which they achieved a 3D organisation and acquired or maintained their differentiation into hepatocytes. Albumin secretion as well as functional bile canaliculi were confirmed on the liver-on-a-chip. Finally, an acetaminophen toxicological assay was performed. With its multiple micro-chambers for hepatocyte culture, this microfluidic device architecture offers a promising opportunity to provide new tools for drug screening applications.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-03286077
Contributor : Bruno Le Pioufle Connect in order to contact the contributor
Submitted on : Thursday, July 15, 2021 - 12:14:39 PM
Last modification on : Tuesday, November 23, 2021 - 3:42:35 AM
Long-term archiving on: : Saturday, October 16, 2021 - 6:27:47 PM

File

s41598-021-92011-7.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Manon Boul, Nassima Benzoubir, Antonietta Messina, Rasta Ghasemi, Ismail Ben Mosbah, et al.. A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation. Scientific Reports, Nature Publishing Group, 2021, 11, pp.14075. ⟨10.1038/s41598-021-92011-7⟩. ⟨hal-03286077⟩

Share

Metrics

Record views

93

Files downloads

99