Efficient quantification of primary amine functions grafted onto apatite ceramics by using two UV-Vis spectrophotometric methods

Vincent Chaleix 1 Chantal Damia 2 Vincent Sol 1, * Eric Champion 2 Evelyne Poli 2
* Auteur correspondant
2 Axe 1 : procédés céramiques
SPCTS - Science des Procédés Céramiques et de Traitements de Surface
Abstract : Enhancement of osteogenic properties of bone substitutes is best achieved by the covalent attachment of appropriate biomolecules, e.g. proteins and peptides, on amino-functionalized implant materials. Measurement of the free amino surface density of these materials is mandatory before derivatization. Two photometric methods were developed to assay the free amino groups of organosilane molecules covalently grafted onto hydroxyapatite and silicon-substituted hydroxyapatite ceramic surfaces. Reaction of amino-derivatized apatites with ninhydrin led to the formation of Ruhemann's purple quantified by the absorbance at 586 nm. Alternatively, ceramics were reacted with Fmoc-derivatized amino acids in the presence of piperidine, leading to the dibenzofulvene–piperidine adduct measured at 301 nm. Both methods allow the detection of free amino groups down to 1.0 × 10−8 mol g−1 of apatite-ceramics and, since they are conducted under non-acid conditions, preserve the acid-sensitive structure of hydroxyapatite.
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https://hal.archives-ouvertes.fr/hal-01086778
Contributeur : Vincent Sol <>
Soumis le : lundi 24 novembre 2014 - 18:55:53
Dernière modification le : mardi 3 juillet 2018 - 10:40:04

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Vincent Chaleix, Chantal Damia, Vincent Sol, Eric Champion, Evelyne Poli. Efficient quantification of primary amine functions grafted onto apatite ceramics by using two UV-Vis spectrophotometric methods. Analytical Methods, Royal Society of Chemistry, 2014, pp.9622-9627. ⟨10.1039/C4AY02012J ⟩. ⟨hal-01086778⟩

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