Deciphering mRNA sequence determinants of protein production rate

Abstract : One of the greatest challenges in biophysical models of translation is to identify coding sequence features that affect the rate of translation and therefore the overall protein production in the cell. We propose an analytic method to solve a translation model based on the inhomogeneous totally asymmetric simple exclusion process, which allows us to unveil simple design principles of nucleotide sequences determining protein production rates. Our solution shows an excellent agreement when compared to numerical genome-wide simulations of S. cerevisiae transcript sequences and predicts that the first 10 codons, which is the ribosome footprint length on the mRNA, together with the value of the initiation rate, are the main determinants of protein production rate under physiological conditions. Finally, we interpret the obtained analytic results based on the evolutionary role of the codons’ choice for regulating translation rates and ribosome densities.
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https://hal.archives-ouvertes.fr/hal-01938431
Contributeur : L2c Aigle <>
Soumis le : mercredi 28 novembre 2018 - 16:25:57
Dernière modification le : jeudi 29 novembre 2018 - 01:18:44

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  • HAL Id : hal-01938431, version 1

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Luca Ciandrini. Deciphering mRNA sequence determinants of protein production rate. Biophysics: Today and Beyond, Apr 2018, Montpellier, France. 〈hal-01938431〉

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