Colonization of non-biodegradable and biodegradable plastics by marine microorganisms

Abstract : Plastics are ubiquitous in the oceans and constitute suitable matrices for bacterial attachment and growth. Understanding biofouling mechanisms is a key issue to assessing the ecological impacts and fate of plastics in marine environment. In this study, we investigated the different steps of plastic colonization of polyolefin-based plastics, on the first one hand, including conventional low-density polyethylene (PE), additivated PE with pro-oxidant (OXO), and artificially aged OXO (AA-OXO); and of a polyester, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), on the other hand. We combined measurements of physical surface properties of polymers (hydrophobicity and roughness) with microbiological characterization of the biofilm (cell counts, taxonomic composition, and heterotrophic activity) using a wide range of techniques, with some of them used for the first time on plastics. Our experimental setup using aquariums with natural circulating seawater during 6 weeks allowed us to characterize the successive phases of primo-colonization, growing, and maturation of the biofilms. We highlighted different trends between polymer types with distinct surface properties and composition, the biodegradable AA-OXO and PHBV presenting higher colonization by active and specific bacteria compared to non-biodegradable polymers (PE and OXO). Succession of bacterial population occurred during the three colonization phases, with hydrocarbonoclastic bacteria being highly abundant on all plastic types. This study brings original data that provide new insights on the colonization of non-biodegradable and biodegradable polymers by marine microorganisms.
Type de document :
Article dans une revue
Frontiers in Microbiology, Frontiers Media, 2018, 9, pp.1571. 〈10.3389/fmicb.2018.01571〉
Liste complète des métadonnées
Contributeur : Stéphanie Bonnefoy <>
Soumis le : jeudi 22 novembre 2018 - 08:27:08
Dernière modification le : samedi 23 mars 2019 - 01:39:25
Document(s) archivé(s) le : samedi 23 février 2019 - 12:51:12


Fichiers éditeurs autorisés sur une archive ouverte



Claire Dussud, Cindy Hudec, Matthieu George, Pascale Fabre, Perry Higgs, et al.. Colonization of non-biodegradable and biodegradable plastics by marine microorganisms. Frontiers in Microbiology, Frontiers Media, 2018, 9, pp.1571. 〈10.3389/fmicb.2018.01571〉. 〈hal-01842397〉



Consultations de la notice


Téléchargements de fichiers