Foci of cyclin A2 interact with actin and RhoA in mitosis

Abdelhalim Loukil , Fanny Izard , Mariya Georgieva , Shaereh Mashayekhan , Jean-Marie Blanchard , Andrea Parmeggiani (1) , Marion Peter

1 L2C - Laboratoire Charles Coulomb

Cyclin A2 is a key player in the regulation of the cell cycle. Its degradation in mid-mitosis depends primarily on the ubiquitin-proteasome system (UPS), while autophagy also contributes. However, a fraction of cyclin A2 persists beyond metaphase. In this work, we focus on cyclin A2-rich foci detected in mitosis by high resolution imaging and analyse their movements. We demonstrate that cyclin A2 interacts with actin and RhoA during mitosis, and that cyclin A2 depletion induces a dramatic decrease in active RhoA in mitosis. Our data suggest cyclin A2 participation in RhoA activation in late mitosis.

Domaines: Biophysique [physics.bio-ph]; Automates cellulaires et gaz sur réseau [nlin.CG]; Adaptation et Systèmes auto-organisés [nlin.AO]; Cancer; Organisation et fonctions cellulaires [q-bio.SC]; Réseaux moléculaires [q-bio.MN]; Physique et Société [physics.soc-ph]

Abdelhalim Loukil, Fanny Izard, Mariya Georgieva, Shaereh Mashayekhan, Jean-Marie Blanchard, et al.. Foci of cyclin A2 interact with actin and RhoA in mitosis. Scientific Reports, 2016, 6, pp.27215. ⟨10.1038/srep27215⟩. ⟨hal-01345990⟩