DIFFRACTION TOMOGRAPHY FROM SINGLE-MOLECULE LOCALIZATION MICROSCOPY: NUMERICAL FEASIBILITY - Signal et Communications Accéder directement au contenu
Communication Dans Un Congrès Année : 2021

DIFFRACTION TOMOGRAPHY FROM SINGLE-MOLECULE LOCALIZATION MICROSCOPY: NUMERICAL FEASIBILITY

Résumé

Single-molecule localization microscopy (SMLM) is a fluorescence microscopy technique that achieves super-resolution imaging by sequentially activating and localizing random sparse subsets of fluorophores. Each activated fluorophore emits light that then scatters through the sample, thus acting as a source of illumination from inside the sample. Hence, the sequence of SMLM frames carries information on the distribution of the refractive index of the sample. In this proof-of-concept work, we explore the possibility of exploiting this information to recover the refractive index of the imaged sample, given the localized molecules. Our results with simulated data suggest that it is possible to exploit the phase information that underlies the SMLM data.
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Dates et versions

hal-03184774 , version 1 (29-03-2021)

Identifiants

  • HAL Id : hal-03184774 , version 1

Citer

Thanh-An Pham, Emmanuel Soubies, Ferréol Soulez, Michael Unser. DIFFRACTION TOMOGRAPHY FROM SINGLE-MOLECULE LOCALIZATION MICROSCOPY: NUMERICAL FEASIBILITY. IEEE 18th International Symposium on Biomedical Imaging (ISBI), Apr 2021, Nice (Virtual), France. ⟨hal-03184774⟩
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