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Regulation of photosynthetic electron flow on dark to light transition by ferredoxin:NADP(H) oxidoreductase interactions

Abstract : During photosynthesis, electron transport is necessary for carbon assimilation and must be regulated to minimize free radical damage. There is a longstanding controversy over the role of a critical enzyme in this process (ferredoxin:NADP(H) oxidoreductase, or FNR), and in particular its location within chloroplasts. Here we use immunogold labelling to prove that FNR previously assigned as soluble is in fact membrane associated. We combined this technique with a genetic approach in the model plant Arabidopsis to show that the distribution of this enzyme between different membrane regions depends on its interaction with specific tether proteins. We further demonstrate a correlation between the interaction of FNR with different proteins and the activity of alternative photosynthetic electron transport pathways. This supports a role for FNR location in regulating photosynthetic electron flow during the transition from dark to light.
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https://hal.archives-ouvertes.fr/hal-03214040
Contributor : Jacqueline Martin-Laffon <>
Submitted on : Friday, April 30, 2021 - 5:47:54 PM
Last modification on : Thursday, May 20, 2021 - 8:48:35 AM

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Manuela Kramer, Melvin Rodriguez-Heredia, Francesco Saccon, Laura Mosebach, Manuel Twachtmann, et al.. Regulation of photosynthetic electron flow on dark to light transition by ferredoxin:NADP(H) oxidoreductase interactions. eLife, eLife Sciences Publication, 2021, 10, ⟨10.7554/eLife.56088⟩. ⟨hal-03214040⟩

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