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Major reorganization of chromosome conformation during muscle development in pig

Abstract : The spatial organization of the genome in the nucleus plays a crucial role in eukaryotic cell functions, yet little is known about chromatin structure variations during late fetal development in mammals. We performed in situ high-throughput chromosome conformation capture (Hi-C) sequencing of DNA from muscle samples of pig fetuses at two late stages of gestation. Comparative analysis of the resulting Hi-C interaction matrices between both groups showed widespread differences of different types. First, we discovered a complex landscape of stable and group-specific Topologically Associating Domains (TADs). Investigating the nuclear partition of the chromatin into transcriptionally active and inactive compartments, we observed a genome-wide fragmentation of these compartments between 90 and 110 days of gestation. Also, we identified and characterized the distribution of differential cis-and trans-pairwise interactions. In particular, trans-interactions at chromosome extremities revealed a mechanism of telomere clustering further confirmed by 3D Fluorescence in situ Hybridization (FISH). Altogether, we report major variations of the three-dimensional genome conformation during muscle development in pig, involving several levels of chromatin remodeling and structural regulation.
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https://hal.inrae.fr/hal-03367056
Contributor : Nathalie Vialaneix Connect in order to contact the contributor
Submitted on : Wednesday, October 6, 2021 - 9:06:05 AM
Last modification on : Friday, October 29, 2021 - 9:14:24 AM

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Distributed under a Creative Commons Attribution 4.0 International License

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Maria Marti-Marimon, Nathalie Vialaneix, Yvette Lahbib Mansais, Matthias Zytnicki, Sylvie Camut, et al.. Major reorganization of chromosome conformation during muscle development in pig. Frontiers in Genetics, Frontiers, 2021, 12, pp.19 P. ⟨10.3389/fgene.2021.748239⟩. ⟨hal-03367056⟩

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