Mechanical response of a megakaryocyte acoustically coupled with a tethered microbubble
Résumé
In recent years, a growing interest arised for using cavitation microbubbles oscillating in steady state for purposes of sonoporation [1]. The mechanisms commonly proposed for cell membrane permeabilization are the creation of liquid flows (microstreaming) and induced shear stresses on the cell membrane [2], as well as significant cell membrane deformation through push/pull phenomena [3]. We here propose an experimental investigation of the interaction between a wall-attached microbubble and a biological cell. The experimental setup consists of a tank filled with cell culture medium. A megakaryocyte (MK, bone marrow cell) with a mean radius ranging between 20 and 30 μm is maintained by a glass capillary. By means of a micromanipulator, a single MK is mechanically coupled with a single wall-attached oscillating microbubble of comparable size, driven by an ultrasound field. An ultra-fast camera captures the time- resolved description of the bubble-cell interaction at a micro-scale level, and hence the characterization of the cell membrane deformations and the bubble interface oscillations. Then, a broad parametric study aims the pairing up of the sonoporation success (by particles internalization) with specific regime of bubble oscillation and modal deformations. First observations seem to indicate that strong cell membrane stretching is correlated to bubble nonspherical deformations. References [1] H.J. Vos, B. Dollet, M. Versluis, and N. de Jong, Nonspherical shape oscillations of coated microbubbles in contact with a wall, Ultrasound in Medicine & Biology, Vol. 37, No. 6, 2011, pp. 935-948. [2] J. Collis, R. Manasseh, P. Liovic, P. Tho, A. Ooi, K. Petkovic-Duran, and Y. Zhu, Cavitation microstreaming and stress fields created by microbubbles, Ultrasonics, Vol. 50, No. 2, 2010, pp. 273-279. [3] Z. Fan, R.E. Kumon, and C.X. Deng, Mechanisms of microbubble-facilitated sonoporation for drug and gene delivery, Therapeutic Delivery, Vol. 5, No. 4, 2014, pp. 467-486.
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