Exploring selective autophagy events in multiple biologic models using LC3-interacting regions (LIR)-based molecular traps

Grégoire Quinet (1) , Pierre Génin (2) , Oznur Ozturk (3) , Naima Belgareh-Touzé (3) , Lilas Courtot (1) , Renaud Legouis (4) , Robert Weil (2) , Mickael Cohen (3) , Manuel S Rodriguez (1)

1 LCC - Laboratoire de chimie de coordination
2 CIMI - Centre d'Immunologie et des Maladies Infectieuses
3 LBMCE - Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
4 I2BC - Institut de Biologie Intégrative de la Cellule

Autophagy is an essential cellular pathway that ensures degradation of a wide range of substrates including damaged organelles or large protein aggregates. Understanding how this proteolytic pathway is regulated would increase our comprehension on its role in cellular physiology and contribute to identify biomarkers or potential drug targets to develop more specific treatments for disease in which autophagy is dysregulated. Here, we report the development of molecular traps based in the tandem disposition of LC3-interacting regions (LIR). The estimated affinity of LC3-traps for distinct recombinant LC3/GABARAP proteins is in the low nanomolar range and allows the capture of these proteins from distinct mammalian cell lines , S. cerevisiae and C. elegans . LC3-traps show preferences for GABARAP/LGG1 or LC3/LGG2 and pull-down substrates targeted to proteaphagy and mitophagy. Therefore, LC3-traps are versatile tools that can be adapted to multiple applications to monitor selective autophagy events in distinct physiologic and pathologic circumstances.

Mots clés: Autophagy; Biochemistry; Macroautophagy; Proteases

Domaines: Biochimie [q-bio.BM]

Grégoire Quinet, Pierre Génin, Oznur Ozturk, Naima Belgareh-Touzé, Lilas Courtot, et al.. Exploring selective autophagy events in multiple biologic models using LC3-interacting regions (LIR)-based molecular traps. Scientific Reports, 2022, 12 (1), pp.7652. ⟨10.1038/s41598-022-11417-z⟩. ⟨hal-03666597⟩