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Université de Provence - Aix-Marseille I Hyper Articles en Ligne |
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Université de Provence - Aix-Marseille I Hyper Articles en Ligne |
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| HAL : halsde-00293033, version 1 |
| DOI : 10.1016/j.resmic.2007.03.004 |
| Fiche détaillée |  Récupérer au format |
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| Research in Microbiology 158, 5 (2007) 413-419 |
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| Phylogenetic analysis of the Aspergillus niger aggregate in relation to feruloyl esterase activity |
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| F. Giraud 1J. Dupont 1 |
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| (2007) |
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| Species of the Aspergillus niger aggregate are known to produce feruloyl esterases, enzymes involved in the degradation of cell wall polymers. However, species delineation is difficult in these fungi. We combined AFLP analysis with ITS rDNA and beta-tubulin sequencing to characterize the isolates of this aggregate in terms of feruloyl esterase production. A preliminary re-examination of isolates based on comparison of ITS rDNA and beta-tubulin sequences with those of typical taxa deposited in international collections led us to re-identify the isolates as members of the species A. niger, A. foetidus and A. tubingensis. Molecular clustering based on beta-tubulin data and AFLP analysis showed that the strains of A. niger formed a homogenous phylogenetic group distinguished by either zero or type A feruloyl esterase activity, while strains A. foetidus and A. tubingensis exhibited type B feruloyl esterase activity when grown on sugar beet pulp. (c) 2007 Elsevier Masson SAS. All rights reserved. |
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| 1 : | Taxonomie - Collections (TC) |
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CNRS : UMS2700 |
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| 2 : | Biotechnologie des Champignons Filamenteux (UBCF) |
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Institut national de la recherche agronomique (INRA) : UR1163 – Université de la Méditerranée - Aix-Marseille II – Université de Provence - Aix-Marseille I |
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| 3 : | Laboratoire d'écologie alpine (LECA) |
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CNRS : UMR5553 – Université Joseph Fourier - Grenoble I – Université de Savoie |
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| domaine | : | Sciences de l'environnement/Biodiversité et Ecologie Sciences du Vivant/Biodiversité Sciences du Vivant/Ecologie, Environnement |
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| Aspergillus niger – feruloyl esterase – phylogeny – beta-tubulin – AFLP – ITS – Length polymorphism analysis – sugar-beet pulp – black aspergilli – section nigri – acid esterases – ochratoxin-a – degradation – aflp – tubingensis – classification |
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| halsde-00293033, version 1 | |
| http://hal-sde.archives-ouvertes.fr/halsde-00293033 | |
| oai:hal-sde.archives-ouvertes.fr:halsde-00293033 | |
| Contributeur : Kim Pla | |
| Soumis le : Jeudi 3 Juillet 2008, 14:52:46 | |
| Dernière modification le : Jeudi 3 Juillet 2008, 15:37:22 | |
