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Université de Provence - Aix-Marseille I Hyper Articles en Ligne |
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Université de Provence - Aix-Marseille I Hyper Articles en Ligne |
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| HAL : hal-00295014, version 1 |
| PubMed : 18604216 |
| DOI : 10.1038/nmeth.1233 |
| Fiche détaillée |  Récupérer au format |
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| Nature Methods 5, 8 (2008) 687-694 |
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| Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes. |
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| Arnauld Sergé 1Nicolas Bertaux 2, 3 |
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| (08/2008) |
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| Although the highly dynamic and mosaic organization of the plasma membrane is well-recognized, depicting a resolved, global view of this organization remains challenging. We present an analytical single-particle tracking (SPT) method and tool, multiple-target tracing (MTT), that takes advantage of the high spatial resolution provided by single-fluorophore sensitivity. MTT can be used to generate dynamic maps at high densities of tracked particles, thereby providing global representation of molecular dynamics in cell membranes. Deflation by subtracting detected peaks allows detection of lower-intensity peaks. We exhaustively detected particles using MTT, with performance reaching theoretical limits, and then reconnected trajectories integrating the statistical information from past trajectories. We demonstrate the potential of this method by applying it to the epidermal growth factor receptor (EGFR) labeled with quantum dots (Qdots), in the plasma membrane of live cells. We anticipate the use of MTT to explore molecular dynamics and interactions at the cell membrane. |
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| 1 : | Centre d'immunologie de Marseille Luminy (CIML) |
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CNRS : UMR6102 – INSERM : U631 – Université de la Méditerranée - Aix-Marseille II |
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| 2 : | Institut FRESNEL (IF) |
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CNRS : UMR6133 – Université de Provence - Aix-Marseille I – Université Paul Cézanne - Aix-Marseille III – Ecole Centrale de Marseille |
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| 3 : | Ecole Centrale de Marseille (ECM) |
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Ecole Centrale de Marseille |
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| Domaine | : | Sciences du Vivant/Immunologie Sciences de l'ingénieur/Traitement du signal et de l'image Informatique/Traitement du signal et de l'image |
| hal-00295014, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00295014 | |
| oai:hal.archives-ouvertes.fr:hal-00295014 | |
| Contributeur : Marie Laure Haon | |
| Soumis le : Vendredi 11 Juillet 2008, 09:12:04 | |
| Dernière modification le : Jeudi 18 Septembre 2008, 21:50:40 | |
