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| HAL : hal-00610579, version 1 |
| PubMed : 21109274 |
| DOI : 10.1016/j.phytochem.2010.10.019 |
| Fiche détaillée |  Récupérer au format |
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| Phytochemistry 72, 10 (2011) 1109-23 |
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| Combining various strategies to increase the coverage of the plant cell wall glycoproteome. |
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| Yu Zhang 1Aurélie Giboulot 1 |
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| (07/2011) |
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| Glycoproteomics recently became a very active field, mostly in mammals. The first part of this paper consists of a mini-review on the strategies used in glycoproteomics, namely methods for enrichment in glycoproteins and mass spectrometry (MS) techniques currently used. In a second part, these strategies are applied to the cell wall glycoproteome of etiolated hypocotyls of Arabidopsis thaliana, showing their complementarity. Several sub-glycoproteomes were obtained by: (i) affinity chromatography on concanavaline A (ConA) and analysis of glycoproteins by MALDI-TOF MS; (ii) multidimensional lectin chromatography (using AIL, PNA, ConA and WGA lectins) and subsequent identification of glycoproteins by MALDI-TOF MS and LC-MS/MS; (iii) boronic acid chromatography followed by identification of glycoproteins by MALDI-TOF MS. Altogether, 127 glycoproteins were identified. Most glycoproteins were found to be putative N-glycoproteins and N-glycopeptides were predicted from MS data using the ProTerNyc bioinformatics software. |
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| 1 : | Surfaces Cellulaires et Signalisation chez les Végétaux (SCSV) |
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CNRS : UMR5546 – Université Paul Sabatier [UPS] - Toulouse III |
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| 2 : | La plante et son environnement (PSE) |
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CNRS : IFR87 – Institut national de la recherche agronomique (INRA) – Institut National Agronomique Paris-Grignon – Université Paris XI - Paris Sud |
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| Domaine | : | Sciences du Vivant/Biologie du développement |
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| plant – glycoproteomics – Arabidopsis – mass spectrometry – ProTerNyc |
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| hal-00610579, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00610579 | |
| oai:hal.archives-ouvertes.fr:hal-00610579 | |
| Contributeur : Elisabeth Jamet | |
| Soumis le : Vendredi 22 Juillet 2011, 15:29:59 | |
| Dernière modification le : Vendredi 22 Juillet 2011, 15:36:27 | |
