 |
|
| HAL : hal-00111673, version 1 |
| DOI : 10.1016/j.bios.2006.05.018 |
| Fiche détaillée |  Récupérer au format |
 |
| Biosensors & bioelectronics 22 (2006) 440-448 |
|
|
|
|
| Building of an immunosensor : how can the composition and structure of the thiol attachment layer affect the immunosensor efficiency ? |
|
|
| Elisabeth Briand 1Michèle Salmain 2 |
|
|
| (27/06/2006) |
|
|
|
| Immunosensors, based on the immobilsation of model rabbit antibodies on a mixed self assembled monolyaers and Protein A as a linking agent on gold transducers, were elaborated and characterised at each step by Modulated polarisation- Infrared Spectroscopy (PM-IRRAS) and punctually by Atomic Force Microscopy (AFM) and Quartz Crystal Microbalance (QCM). By testing two different mixed SAM's layers, mercaptoundecanoic acid (MUA), together with either decanethiol or mercaptohexanol, the role of the chemical compostion and structure of the antibody attachment layer upon the sensor performance was demonstrated. |
|
|
|
|
|
|
|
|
|
|
|
| 1 : | Laboratoire de Réactivité de Surface (LRS) |
|
|
|
CNRS : UMR7197 – Université Pierre et Marie Curie (UPMC) - Paris VI |
|
|
| 2 : | Chimie et biochimie des complexes moléculaires (CBCM) |
|
|
|
CNRS : UMR7576 – Ecole Nationale Supérieure de Chimie de Paris |
|
|
| 3 : | Unité de Recherche en Bioadhésion et Hygiène des Matériaux |
|
|
|
Institut national de la recherche agronomique (INRA) |
|
|
| 4 : | Laboratoire Interfaces et Systèmes Electrochimiques (LISE) |
|
|
|
CNRS : UPR15 – Université Pierre et Marie Curie (UPMC) - Paris VI |
|
|
| 5 : | Interfaces et capteurs |
|
|
|
Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
|
|
|
|
|
|
|
|
|
| Domaine | : | Chimie/Catalyse Sciences du Vivant/Biotechnologies Informatique/Biotechnologie |
|
|
| immunosensors – SAMs |
|
| hal-00111673, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00111673 | |
| oai:hal.archives-ouvertes.fr:hal-00111673 | |
| Contributeur : Claire-Marie Pradier | |
| Soumis le : Lundi 6 Novembre 2006, 10:11:51 | |
| Dernière modification le : Mercredi 30 Mars 2011, 15:39:37 | |
