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| HAL: inserm-00643082, version 1 |
| PubMed: 22094473 |
| DOI: 10.1152/ajpendo.00314.2011 |
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| AJP Endocrinology and Metabolism / American Journal of Physiology - Endocrinology and Metabolism 302, 3 (2012) E374-86 |
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| Oil composition of high-fat diet affects metabolic inflammation differently in connection with endotoxin receptors in mice. |
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| Fabienne Laugerette 1Jean-Pierre Furet 2 |
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| (2012-02) |
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| Low-grade inflammation observed in obesity is a risk factor for cardiovascular disease. Recent studies revealed that this would be linked to gut-derived endotoxemia during fat digestion in high-fat diets, but nothing is known about the effect of lipid composition. The study was designed to test the impact of oil composition of high-fat diets on endotoxin metabolism and inflammation in mice. C57/Bl6 mice were fed for 8 wk with chow or isocaloric isolipidic diets enriched with oils differing in fatty acid composition: milk fat, palm oil, rapeseed oil, or sunflower oil. In vitro, adipocytes (3T3-L1) were stimulated or not with lipopolysaccharide (LPS; endotoxin) and incubated with different fatty acids. In mice, the palm group presented the highest level of IL-6 in plasma (P < 0.01) together with the highest expression in adipose tissue of IL-1β and of LPS-sensing TLR4 and CD14 (P < 0.05). The higher inflammation in the palm group was correlated with a greater ratio of LPS-binding protein (LBP)/sCD14 in plasma (P < 0.05). The rapeseed group resulted in higher sCD14 than the palm group, which was associated with lower inflammation in both plasma and adipose tissue despite higher plasma endotoxemia. Taken together, our results reveal that the palm oil-based diet resulted in the most active transport of LPS toward tissues via high LBP and low sCD14 and the greatest inflammatory outcomes. In contrast, a rapeseed oil-based diet seemed to result in an endotoxin metabolism driven toward less inflammatory pathways. This shows that dietary fat composition can contribute to modulate the onset of low-grade inflammation through the quality of endotoxin receptors. |
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| 1: | Laboratoire de recherche en cardiovasculaire, métabolisme, diabétologie et nutrition (CARMEN) |
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INSERM : U1060 – Institut national de la recherche agronomique (INRA) – Institut National des Sciences Appliquées (INSA) - Lyon – Université Claude Bernard - Lyon I – Hospices Civils de Lyon |
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| 2: | MICrobiologie de l'ALImentation au Service de la Santé humaine (MICALIS) |
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Institut national de la recherche agronomique (INRA) : UMR1319 – AgroParisTech |
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| 3: | Institut Multidisciplinaire de Biochimie des Lipides (IMBL) |
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Institut National des Sciences Appliquées (INSA) - Lyon |
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| 4: | Universite Claude Bernard - Lyon I |
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Université Claude Bernard - Lyon I |
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| 5: | Centre de Recherche en Nutrition Humaine Rhône-Alpes (CRNH) |
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Institut national de la recherche agronomique (INRA) – CHU Grenoble – Université Claude Bernard - Lyon I – Hospices Civils de Lyon – INSERM – CHU Saint-Etienne – Université Jean Monnet - Saint-Etienne – Université Joseph Fourier - Grenoble I |
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| subject | : | Life Sciences/Food and Nutrition |
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| Fatty acid – endotoxin – sCD14 – LBP – nutrition |
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| Attached file list to this document: | |||||
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| inserm-00643082, version 1 | |
| http://www.hal.inserm.fr/inserm-00643082 | |
| oai:www.hal.inserm.fr:inserm-00643082 | |
| From: Evelyne Vericel | |
| Submitted on: Wednesday, 21 November 2012 07:00:30 | |
| Updated on: Wednesday, 21 November 2012 07:00:30 | |
