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Chemical Geology 265, 1-2 (2009) 54-62
Experimental approach of CO2 biomineralization in deep saline aquifers
Sebastien Dupraz 1, Benedicte Menez 1, Philippe Gouze 2, Richard Leprovost 2, Pascale Benezeth 3, Oleg S. Pokrovsky 3, Francois Guyot 1
(2009)

We describe an experimental system including monitoring of temperature, pressure, pH, oxidation reduction potential and optical density at 600 nm, designed for studying the role of microorganisms on the geological sequestration Of CO2 and its transformation into solid carbonate phases. Measurements were performed in an artificial ground water (AGW) supplemented with urea (2 g.l(-1)) and equilibrated at controlled temperatures with a gaseous phase before bacterial inoculation. We used the ureolytic strain Bacillus pasteurii as a model carbonate precipitating bacteria and showed that it can successfully promote strong pH increases by ureolysis in the AGW equilibrated with CO2 pressures of up to I bar. Increasing salinities (5.8,13.5 and 35.0 g.l(-1)) have a positive effect on the rate of pH increase, whereas the effect of increasing temperatures (30,35 and 38 degrees C) is less important. Calcium is also shown to have a specific positive influence on the rate of ureolysis. The number of viable cells present in solution decreases greatly during the carbonate precipitation event but the population partially recovers once precipitation is over.
1 :  Institut de Physique du Globe de Paris (IPGP)
CNRS : UMR7154 – INSU – IPG PARIS – Université Pierre et Marie Curie [UPMC] - Paris VI – Université Paris VII - Paris Diderot – Université de la Réunion
2 :  Géosciences Montpellier (GM)
CNRS : UMR5243 – Université Montpellier II - Sciences et techniques
3 :  Laboratoire des Mécanismes et Transfert en Géologie (LMTG)
CNRS : UMR5563 – Observatoire Midi-Pyrénées – Université Paul Sabatier [UPS] - Toulouse III – Institut de recherche pour le développement [IRD] : UMR154
Planète et Univers/Interfaces continentales, environnement

Sciences de l'environnement/Milieux et Changements globaux
CO2 geological storage – Biomineralization – Carbonate precipitation – Bacillus pasteurii – Sporosarcina pasteurii – Ureolysis – Calcium bioassimilation – Geomicrobiology – Bacteria/mineral interactions