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Plant and Soil 319, 1-2 (2009) 1-13
Root exclusion through trenching does not affect the isotopic composition of soil CO2 efflux
Nicolas Chemidlin Prévost-Bouré 1, Jérome Ngao 1, Daniel Berveiller 1, D. Bonal 2, Claire Damesin 1, Eric Dufrene 1, Jean-Christophe Lata 3, Valérie Le Dantec 4, B. Longdoz 5, Stéphane Ponton 5, Kamel Soudani 1, Daniel Epron 6
(01/06/2009)

Disentangling the autotrophic and heterotrophic components of soil CO2 efflux is critical to understanding the role of soil system in terrestrial carbon (C) cycling. In this study, we combined a stable C-isotope natural abundance approach with the trenched plot method to determine if root exclusion significantly affected the isotopic composition (δ13C) of soil CO2 efflux (RS). This study was performed in different forest ecosystems: a tropical rainforest and two temperate broadleaved forests, where trenched plots had previously been installed. At each site, RS and its δ13C (δ13CRs) tended to be lower in trenched plots than in control plots. Contrary to RS, δ13CRs differences were not significant. This observation is consistent with the small differences in δ13C measured on organic matter from root, litter and soil. The lack of an effect on δ13CRs by root exclusion could be from the small difference in δ13C between autotrophic and heterotrophic soil respirations, but further investigations are needed because of potential artefacts associated with the root exclusion technique.
1 :  Ecologie, Systématique et Evolution (ESE)
CNRS : UMR8079 – Université Paris XI - Paris Sud – Ecole Nationale du Génie Rural des Eaux et Forêts
2 :  UMR-ECOFOG
UMR-ECOFOG
3 :  Biogéochimie et écologie des milieux continentaux (Bioemco)
CNRS : UMR7618 – Université Pierre et Marie Curie [UPMC] - Paris VI – Université Paris-Est Créteil Val-de-Marne (UPEC) – Ecole normale supérieure de Paris - ENS Paris – Institut de recherche pour le développement [IRD] – AgroParisTech – Institut national de la recherche agronomique (INRA) : UMR1122
4 :  Centre d'études spatiales de la biosphère (CESBIO)
CNRS : UMR5126 – Institut de recherche pour le développement [IRD] – CNES – Observatoire Midi-Pyrénées – INSU – Université Paul Sabatier [UPS] - Toulouse III
5 :  Ecologie et Ecophysiologie Forestières (EEF)
Institut national de la recherche agronomique (INRA) : UR1137 – Université Henri Poincaré - Nancy I
6 :  Laboratoire de Biologie et Ecophysiologie
Université de Franche-Comté
Sciences de l'environnement/Biodiversité et Ecologie
Stable carbon isotopes – Natural abundance – Soil respiration – Trenched plot – Rainforest – Temperate forest