| Type de publication : |
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Articles dans des revues avec comité de lecture |
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| Domaine : |
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Sciences du Vivant/Biologie cellulaire
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| Titre : |
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Identification of potential cellular targets of aloisine A by affinity chromatography. |
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| Auteur(s) : |
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Caroline Corbel 1, Rose Haddoub, Damien Guiffant, Olivier Lozach 2, David Gueyrard, Jérôme Lemoine 3, Morgane Ratin, Laurent Meijer 2, Stéphane Bach, Peter Goekjian 4 |
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| Laboratoire : |
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| Résumé : |
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Affinity chromatography was used to identify potential cellular targets of aloisine A (7-n-butyl-6-(4'-hydroxyphenyl)-5H-pyrrolo[2,3b]pyrazine), a potent inhibitor of cyclin-dependent kinases. This technique is based on the immobilization of the drug on a solid matrix, followed by identification of specifically bound proteins. To this end, both aloisine A and the protein-kinase inactive control N-methyl aloisine, bearing extended linker chains have been synthesized. We present the preparation of such analogues having the triethylene glycol chain at different positions of the molecule, as well as their immobilization on an agarose-based matrix. Affinity chromatography of various biological extracts on the aloisine matrices allowed the identification of both protein kinases and non-kinase proteins as potential cellular targets of aloisine. |
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Langue du texte
intégral : |
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Anglais |
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| Journal : |
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Bioorg Med Chem |
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| Audience : |
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internationale |
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| Date de publication : |
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01/08/2009 |
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Date de publication
électronique : |
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18/06/2009 |
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| Volume : |
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17 |
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| Numéro : |
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15 |
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| Page, identifiant, ... : |
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5572-82 |
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