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Observatoire Midi-Pyrénées |  |
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Observatoire Midi-Pyrénées | |
| HAL: hal-00416033, version 1 |
| PubMed: 19596197 |
| DOI: 10.1016/j.bmc.2009.06.024 |
| Detailed view |  Export this paper |
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| Bioorg Med Chem 17, 15 (2009) 5572-82 |
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| Identification of potential cellular targets of aloisine A by affinity chromatography. |
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| Caroline Corbel 1Rose Haddoub |
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| (2009-08-01) |
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| Affinity chromatography was used to identify potential cellular targets of aloisine A (7-n-butyl-6-(4'-hydroxyphenyl)-5H-pyrrolo[2,3b]pyrazine), a potent inhibitor of cyclin-dependent kinases. This technique is based on the immobilization of the drug on a solid matrix, followed by identification of specifically bound proteins. To this end, both aloisine A and the protein-kinase inactive control N-methyl aloisine, bearing extended linker chains have been synthesized. We present the preparation of such analogues having the triethylene glycol chain at different positions of the molecule, as well as their immobilization on an agarose-based matrix. Affinity chromatography of various biological extracts on the aloisine matrices allowed the identification of both protein kinases and non-kinase proteins as potential cellular targets of aloisine. |
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| 1: | Laboratoire des Solides Irradiés (LSI) |
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CNRS : UMR7642 – Polytechnique - X – CEA : DSM/IRAMIS |
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| 2: | Molécules et cibles thérapeutiques (MCT) |
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CNRS : UPS2682 |
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| 3: | Dynamique terrestre et planétaire (DTP) |
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CNRS : UMR5562 – Observatoire Midi-Pyrénées – INSU – Université Paul Sabatier [UPS] - Toulouse III |
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| 4: | inconnu |
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Inconnu |
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| Subject | : | Life Sciences/Cellular Biology |
| hal-00416033, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00416033 | |
| oai:hal.archives-ouvertes.fr:hal-00416033 | |
| From: Allison Favey | |
| Submitted on: Friday, 11 September 2009 16:40:41 | |
| Updated on: Friday, 11 September 2009 16:40:41 | |
