| Publication type: |
 |
Article in peer-reviewed journal |
|
| Subject: |
|
Life Sciences/Biochemistry, Molecular Biology/Biophysics
|
|
| Title: |
|
Wrapping nanocrystals with an amphiphilic polymer preloaded with fixed amounts of fluorophore generates FRET-based nanoprobes with a controlled donor/acceptor ratio. |
|
| Author(s): |
|
Aleksey V Yakovlev 1, Feng Zhang 2, Ali Zulqurnain, Abbasi Azhar-Zahoor, Camilla Luccardini 3, Stéphane Gaillard 4, Jean-Maurice Mallet 5, Patrick Tauc 6, Jean-Claude Brochon 6, Wolfgang J Parak, Anne Feltz 7, Martin Oheim 7 |
|
| Laboratory: |
|
|
|
| Abstract: |
|
Colloidal nanocrystal (NC) donors wrapped with a polymer coating including multiple organic acceptor molecules are promising scaffolds for fluorescence resonance energy transfer (FRET)-based nanobiosensors. Over other self-assembling donor-acceptor configurations, our preloaded polymers have the virtue of producing compact assemblies with a fixed donor/acceptor distance. This property, together with the possibility of stoichiometric polymer loading, allowed us to directly address how the FRET efficiency depended on the donor/acceptor. At the population level, nanoprobes based on commercial as well as custom CdSe/ZnS donors displayed the expected dose-dependent rise in transfer efficiency, saturating from about five ATTO dyes/NC. However, for a given acceptor concentration, both the intensity and lifetime of single-pair FRET data revealed a large dispersion of transfer efficiencies, highlighting an important heterogeneity among nominally identical FRET-based nanoprobes. Rigorous quality check during synthesis and shell assembly as well as postsynthesis sorting and purification are required to make hybrid semiconductor-organic nanoprobes a robust and viable alternative to organic or genetically encoded nanobiosensors. |
|
| Fulltext language: |
|
English |
|
|
| Journal: |
|
| Langmuir (Langmuir) |
| Publisher |
American Chemical Society |
| ISSN |
0743-7463 (eISSN : 1520-5827) |
|
|
| Audience: |
|
international |
|
| Publication date: |
|
2009-03-03 |
|
| Volume: |
|
25 |
|
| Issue: |
|
5 |
|
| Page, identifiant, ...: |
|
3232-9 |
|
|
| MeSH Classification: |
|
Cyclohexanes – Diffusion – Emulsions – Ethanol – Fluorescence Resonance Energy Transfer – Hydrolysis – Kinetics – Magnetic Resonance Spectroscopy – Micelles – Microscopy – Electron – Transmission – Models – Statistical – Nanoparticles – Nanotechnology – Oils – Temperature |
|
|
Export this paper
