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| HAL : hal-00542531, version 1 |
| PubMed : 12719268 |
| PubMed Central : 1302899 |
| DOI : 10.1016/S0006-3495(03)70063-0 |
| Fiche détaillée |  Récupérer au format |
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| Biophysical Journal 84, 5 (2003) 3414-24 |
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| Analysis of cell kinetics using a cell division marker: mathematical modeling of experimental data. |
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| Samuel Bernard 1Laurent Pujo-Menjouet 2 |
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| (05/2003) |
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| We consider an age-maturity structured model arising from a blood cell proliferation problem. This model is "hybrid", i.e., continuous in time and age but the maturity variable is discrete. This is due to the fact that we include the cell division marker carboxyfluorescein diacetate succinimidyl ester. We use our mathematical analysis in conjunction with experimental data taken from the division analysis of primitive murine bone marrow cells to characterize the maturation/proliferation process. Cell cycle parameters such as proliferative rate beta, cell cycle duration tau, apoptosis rate gamma, and loss rate micro can be evaluated from CarboxyFluorescein diacetate Succinimidyl Ester + cell tracking experiments. Our results indicate that after three days in vitro, primitive murine bone marrow cells have parameters beta = 2.2 day(-1), tau = 0.3 day, gamma = 0.3 day(-1), and micro = 0.05 day(-1). |
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| 1 : | Institut Camille Jordan (ICJ) |
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CNRS : UMR5208 – Université Claude Bernard - Lyon I – Ecole Centrale de Lyon – Institut National des Sciences Appliquées (INSA) - Lyon |
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| 2 : | Department of Mathematics, Vanderbilt University |
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Vanderbilt University of Nashville |
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| 3 : | Centre for Nonlinear Dynamics (CND) |
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McGill University |
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| Domaine | : | Mathématiques/Systèmes dynamiques Sciences du Vivant/Bio-Informatique, Biologie Systémique Informatique/Bio-informatique |
| hal-00542531, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00542531 | |
| oai:hal.archives-ouvertes.fr:hal-00542531 | |
| Contributeur : Samuel Bernard | |
| Soumis le : Jeudi 2 Décembre 2010, 20:59:03 | |
| Dernière modification le : Jeudi 2 Décembre 2010, 20:59:03 | |
