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| HAL : hal-00375681, version 1 |
| arXiv : 0904.2648 |
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| Comparison of the photoluminescence properties of semiconductor quantum dots and non-blinking diamond nanoparticles. Observation of the diffusion of diamond nanoparticles in living cells. |
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| Orestis Faklaris 1Damien Garrot 1 |
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| (09/04/2009) |
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| Long-term observations of photoluminescence at the single-molecule level were until recently very diffcult, due to the photobleaching of organic fluorophore molecules. Although inorganic semiconductor nanocrystals can overcome this diffculty showing very low photobleaching yield, they suffer from photoblinking. A new marker has been recently introduced, relying on diamond nanoparticles containing photoluminescent color centers. In this work we compare the photoluminescence of single quantum dots (QDs) to the one of nanodiamonds containing a single-color center. Contrary to other markers, photoluminescent nanodiamonds present a perfect photostability and no photoblinking. At saturation of their excitation, nanodiamonds photoluminescence intensity is only three times smaller than the one of QDs. Moreover, the bright and stable photoluminescence of nanodiamonds allows wide field observations of single nanoparticles motion. We demonstrate the possibility of recording the tra jectory of such single particle in culture cells. |
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| 1 : | Laboratoire de Photonique Quantique et Moléculaire (LPQM) |
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CNRS : UMR8537 – École normale supérieure de Cachan - ENS Cachan |
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| 2 : | Structure et activité des biomolécules normales et pathologiques |
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INSERM : U829 – Université d'Evry-Val d'Essonne |
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| 3 : | Laboratoire de mécanique des sols, structures et matériaux (MSSMat) |
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CNRS : UMR8579 – Ecole Centrale Paris |
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| 4 : | Conditions Extrêmes et Matériaux : Haute Température et Irradiation (CEMHTI) |
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CNRS : UPR3079 – Université d'Orléans |
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| Domaine | : | Physique/Physique/Optique Physique/Physique Quantique Physique/Matière Condensée/Science des matériaux |
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| quantum dots – nanodiamonds – biomarker – photoluminescence – photostability |
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| hal-00375681, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00375681 | |
| oai:hal.archives-ouvertes.fr:hal-00375681 | |
| Contributeur : François Treussart | |
| Soumis le : Mercredi 15 Avril 2009, 22:49:36 | |
| Dernière modification le : Vendredi 17 Avril 2009, 09:33:39 | |
