| Type de publication : |
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Articles dans des revues avec comité de lecture |
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| Domaine : |
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Sciences du Vivant/Biochimie, Biologie Moléculaire
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| Titre : |
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Repression of transcription at the human T-cell receptor Vbeta2.2 segment is mediated by a MAX/MAD/mSin3 complex acting as a scaffold for HDAC activity. |
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| Auteur(s) : |
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Marie-Pierre Font 1, Myriam Cubizolles 2, Hervé Dombret 1, Lucien Cazes 1, Virginie Brenac 2, François Sigaux 1, Malcolm Buckle ( ) 3 |
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| Laboratoire : |
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| Résumé : |
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The identification of protein components in complex networks of co-regulators responsible for the modulation of proliferation versus differentiation modes of cell growth is a major problem. We use a combination of surface enhanced laser desorption/ionization mass spectrometry, surface plasmon resonance coupled to electrospray mass spectrometry, and immunoelectromobility shift assays to identify members of the MAX/MAD family binding to a specific DNA silencer fragment involved in the regulation of transcription for the human T-cell receptor Vbeta2.2 segment. We also identify the cofactors mSin3 and N-CoR known to interact with histone deacetylases. Inhibition of deacetylase activity in Jurkat cells prevented transcription inhibitor complex formation at the Vbeta2.2 segment, suggesting that this is either directly or indirectly dependent on the presence of HDACs. |
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Langue du texte
intégral : |
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Anglais |
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| Journal : |
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Biochemical and Biophysical Research Communications / Biochemistry and Biophysics Research Communications |
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| Audience : |
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non spécifiée |
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| Date de publication : |
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17/12/2004 |
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| Volume : |
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325 |
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| Numéro : |
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3 |
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| Page, identifiant, ... : |
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1021-9 |
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| Descripteur(s) MeSH : |
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DNA-Binding Proteins – Down-Regulation – Gene Expression Regulation – Histone Deacetylases – Humans – Jurkat Cells – Promoter Regions (Genetics) – Receptors – Antigen – T-Cell – alpha-beta – Repressor Proteins – Transcription – Genetic |
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