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| HAL: hal-00599949, version 1 |
| DOI: 10.1021/ac7016635 |
| Detailed view |  Export this paper |
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| Analytical Chemistry 80, 1 (2008) 328-332 |
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| Lipid Bilayer Microarray for Parallel Recording of Transmembrane Ion Currents |
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| B. Le Pioufle 1, 2H. Suzuki 3 |
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| (2008) |
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| This paper describes a multiwell biochip for simultaneous parallel recording of ion current through transmembrane pores reconstituted in planar lipid bilayer arrays. Use of a thin poly(p-xylylene) (parylene) film having micrometer-sized apertures (φ = 15−50 μm, t = 20 μm) led to formation of highly stable bilayer lipid membranes (BLMs) for incorporation of transmembrane pores; thus, a large number of BLMs could be arrayed without any skillful technique. We optically confirmed the simultaneous formation of BLMs in a 5 × 5 matrix, and in our durability test, the BLM lasted more than 15 h. Simultaneous parallel recording of alamethicin and gramicidin transmembrane pores in multiple contiguous recording sites demonstrated the feasibility of high-throughput screening of transmembrane ion currents in artificial lipid bilayers. |
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| 1: | Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE) |
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CNRS : UMR8029 – École normale supérieure de Cachan - ENS Cachan |
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| 2: | LIMMS (UMI2820) |
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CNRS : UMI2820 |
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| 3: | Institute of Industrial Sciences (IIS) |
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University of Tokyo |
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| 4: | Osaka University |
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Okaka University |
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| hal-00599949, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00599949 | |
| oai:hal.archives-ouvertes.fr:hal-00599949 | |
| From: Bruno Le Pioufle | |
| Submitted on: Saturday, 11 June 2011 20:40:43 | |
| Updated on: Thursday, 7 March 2013 22:50:58 | |
