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| HAL: hal-00421164, version 1 |
| PubMed: 19437725 |
| Detailed view |  Export this paper |
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| Langmuir 25, 5 (2009) 3232-9 |
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| Wrapping nanocrystals with an amphiphilic polymer preloaded with fixed amounts of fluorophore generates FRET-based nanoprobes with a controlled donor/acceptor ratio. |
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| Aleksey V Yakovlev 1Feng Zhang 2 |
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| (2009-03-03) |
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| Colloidal nanocrystal (NC) donors wrapped with a polymer coating including multiple organic acceptor molecules are promising scaffolds for fluorescence resonance energy transfer (FRET)-based nanobiosensors. Over other self-assembling donor-acceptor configurations, our preloaded polymers have the virtue of producing compact assemblies with a fixed donor/acceptor distance. This property, together with the possibility of stoichiometric polymer loading, allowed us to directly address how the FRET efficiency depended on the donor/acceptor. At the population level, nanoprobes based on commercial as well as custom CdSe/ZnS donors displayed the expected dose-dependent rise in transfer efficiency, saturating from about five ATTO dyes/NC. However, for a given acceptor concentration, both the intensity and lifetime of single-pair FRET data revealed a large dispersion of transfer efficiencies, highlighting an important heterogeneity among nominally identical FRET-based nanoprobes. Rigorous quality check during synthesis and shell assembly as well as postsynthesis sorting and purification are required to make hybrid semiconductor-organic nanoprobes a robust and viable alternative to organic or genetically encoded nanobiosensors. |
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| 1: | School of EECE |
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University of Newcastle upon Tyne |
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| 2: | Institute of Low Energy Nuclear Physics |
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Beijing Normal University / Beijing |
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| 3: | Laboratoire Kastler Brossel (LKB (Lhomond)) |
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CNRS : UMR8552 – Université Pierre et Marie Curie [UPMC] - Paris VI – Ecole normale supérieure de Paris - ENS Paris |
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| 4: | Institut de Mathématiques de Bordeaux (IMB) |
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CNRS : UMR5251 – Université Sciences et Technologies - Bordeaux I – Université Victor Segalen - Bordeaux II |
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| 5: | BIOMOLÉCULES : SYNTHÈSE, STRUCTURE ET MODE D'ACTION (UMR CNRS 8642.) |
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Ecole Normale Suprieure, |
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| 6: | Laboratoire de Biotechnologie et Pharmacologie génétique Appliquée (LBPA) |
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CNRS : UMR8113 – École normale supérieure de Cachan - ENS Cachan |
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| 7: | Neurobiologie cellulaire et moléculaire (NCM) |
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CNRS : UMR8544 – Ecole normale supérieure de Paris - ENS Paris |
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| Subject | : | Life Sciences/Biochemistry, Molecular Biology/Biophysics |
| hal-00421164, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00421164 | |
| oai:hal.archives-ouvertes.fr:hal-00421164 | |
| From: Patrick Tauc | |
| Submitted on: Thursday, 1 October 2009 00:27:32 | |
| Updated on: Thursday, 1 October 2009 00:27:32 | |
