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| HAL: hal-00162079, version 1 |
| DOI: 10.1016/j.ab.2007.04.001 |
| Detailed view |  Export this paper |
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| Analytical Biochemistry 367 (2007) 95-103 |
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| Assessment of cellular actin dynamics by measurement of fluorescence anisotropy |
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| Jean-Alexis Spitz 1, 2Valérie Polard 2, 3 |
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| (2007) |
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| To study cellular actin dynamics, a cell-free assay based on fluorescence anisotropy was developed. Using G-actin-Alexa as a probe, we found that anisotropy enhancement reflects F-actin elongation. Anisotropy enhancement varies with the concentration of magnesium and calcium cations and with ethylenediaminetetraacetate or well-known effectors of the polymerization. This assay gives the overall status of actin dynamics in cell extracts which are the closest conditions to in vivo, implying most of the regulating proteins that are missing in purified actin measurements. It can be used in a large-scale screening for chemical compounds which modulate actin polymerization. |
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| 1: | Laboratoire de Photophysique et Photochimie Supramoléculaires et Macromoléculaires (PPSM) |
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CNRS : UMR8531 – École normale supérieure de Cachan - ENS Cachan |
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| 2: | Institut d'Alembert (IA) |
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École normale supérieure de Cachan - ENS Cachan – CNRS : IFR121 |
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| 3: | Laboratoire de Biotechnologie et Pharmacologie génétique Appliquée (LBPA) |
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CNRS : UMR8113 – École normale supérieure de Cachan - ENS Cachan |
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| Subject | : | Physics/Physics/Chemical Physics |
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| Actin cytoskeleton – Fluorescence anisotropy – Polymerization |
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| hal-00162079, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00162079 | |
| oai:hal.archives-ouvertes.fr:hal-00162079 | |
| From: Jean-Jacques Vachon | |
| Submitted on: Thursday, 12 July 2007 12:33:37 | |
| Updated on: Tuesday, 18 December 2007 14:46:06 | |
