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| HAL: hal-00598629, version 1 |
| PubMed: 21213259 |
| DOI: 10.1002/bip.21583 |
| Detailed view |  Export this paper |
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| Biopolymers / Biopolymers (Biospectroscopy); Biopolymers (Pept Sci ); Biopolymers (Peptide Sci ); Biopolymers (Pept Sci); Biopolymers 95, 6 (2011) 365-375 |
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| Liquid crystal phases of DNA: Evaluation of DNA organization by two-photon fluorescence microscopy and polarization analysis. |
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Joanna Olesiak-Bańska 1Halina Mojzisova 2 |
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| (2011-01-06) |
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| We report on the investigation of the structure of DNA liquid crystal (LC) phases by means of polarization sensitive two-photon microscopy (PSTPM). DNA was stained with fluorescent dyes, an intercalator propidium iodide or a groove binder Hoechst 3342 and the angular dependence of the intensity of two-photon excited fluorescence (TPEF) emitted by the dye was collected. The local orientation of DNA molecules in cholesteric and columnar liquid crystal phases was established on the basis of the relative angle between the transition dipole of the dye and the long axis of DNA helix. Three dimensional images of the cholesteric phase were obtained making use of the intrinsic 3D resolving ability of two-photon microscopy. We also discuss the influence of dyes on the parameters of DNA LC phases and comment on advantages and limitations of the PSTPM technique in comparison with other LC characterization techniques. © 2011 Wiley Periodicals, Inc. Biopolymers, 2011. |
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| 1: | Institute of Physical and Theoretical Chemistry |
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"Wroclaw University of Technology |
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| 2: | Laboratoire de Photonique Quantique et Moléculaire (LPQM) |
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CNRS : UMR8537 – École normale supérieure de Cachan - ENS Cachan |
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| 3: | Laboratoire de Biotechnologie et Pharmacologie génétique Appliquée (LBPA) |
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CNRS : UMR8113 – École normale supérieure de Cachan - ENS Cachan |
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| Subject | : | Life Sciences/Biochemistry, Molecular Biology/Biophysics |
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| DNA liquid crystal – polarization sensitive two-photon microscopy – propidium iodide – Hoechst 3342 – cholesteric phase – columnar phase – two-photon excited fluorescence |
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| hal-00598629, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00598629 | |
| oai:hal.archives-ouvertes.fr:hal-00598629 | |
| From: Patrick Tauc | |
| Submitted on: Tuesday, 7 June 2011 10:56:40 | |
| Updated on: Sunday, 19 June 2011 20:19:00 | |
