 |
 |
|
|
| HAL : inserm-00276758, version 1 |
| PubMed : 17477444 |
| DOI : 10.1002/mabi.200600263 |
| Fiche détaillée |  Récupérer au format |
 |
| Macromolecular Bioscience 7, 5 (2007) 599-610 |
|
|
|
|
| Evaluation of endothelial cell adhesion onto different protein/gold electrodes by EIS. |
|
|
| Amira Bouafsoun 1, 2Saloua Helali 1 |
|
|
| (10/05/2007) |
|
|
|
| To study cell attachment to biomaterials, several proteins such as fibronectin, collagen IV, heparin, immunoglobulin G, and albumin have been deposited onto polystyrene adsorbed on a self-assembled monolayer (silane or thiol) on glass or gold, respectively. The different steps of this multilayer assembly have been characterized by electrochemical impedance spectroscopy (EIS). These data are compared to those of adhesion rate, viability percentage, and cytoskeleton labeling for a better understanding of the cell adhesion process to each protein. All the proteins are endothelial cell adhering biomolecules but not with the same features. A linear relationship has been established between adhesion rate and resistance of the endothelial cell/protein interface for all negatively charged proteins. |
|
|
|
|
|
|
|
|
|
|
|
| 1 : | Centre de génie électrique de Lyon (CEGELY) |
|
|
|
CNRS : UMR5005 – Université Claude Bernard - Lyon I – Institut National des Sciences Appliquées (INSA) - Lyon – Ecole Centrale de Lyon |
|
|
| 2 : | Laboratoire de biophysique |
|
|
|
Faculté de Médecine de Monastir |
|
|
| 3 : | Physiopathologie des Lipides et Membranes (PLM) |
|
|
|
INSERM : U585 – Institut National des Sciences Appliquées (INSA) - Lyon |
|
|
| 4 : | Sciences Analytiques (SA) |
|
|
|
CNRS : UMR5180 – Université Claude Bernard - Lyon I |
|
|
|
|
|
|
|
|
|
| domaine | : | Sciences du Vivant/Alimentation et Nutrition |
| inserm-00276758, version 1 | |
| http://www.hal.inserm.fr/inserm-00276758 | |
| oai:www.hal.inserm.fr:inserm-00276758 | |
| Contributeur : Evelyne Vericel | |
| Soumis le : Vendredi 2 Mai 2008, 09:08:56 | |
| Fichier(s) disponible le : document non public | |
| Dernière modification le : Lundi 5 Mai 2008, 10:57:26 | |
